In the past year, we have developed software for phosphoproteomic site-assignment and for clustering of dynamic data (in preparation for publication). We have also developed and published software to aid in the design of peptide-directed and fusion protein-directed antibodies called NHLBI-AbDesigner (http://helixweb.nih.gov/AbDesigner/). A paper describing this tool and illustrating its use has been published (Pisitkun et al. NHLBI-AbDesigner: An online tool for design of peptide-directed antibodies. American Journal of Physiology: Cell Physiology. Epub in advance of printing, 2011). We have also developed software to produce sequence logos from phosphoproteomic data called Phosphologo (in review). A list of available software programs can be found at http://helixweb.nih.gov/ESBL/. Finally, we have maintained an improved our proteomics and transcriptomic databases. A list of these databases can be found online at http://helixweb.nih.gov/ESBL/Database/index.html.

Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2011
Total Cost
$157,909
Indirect Cost
Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
Zip Code
LeMaire, Sophia M; Raghuram, Viswanathan; Grady, Cameron R et al. (2017) Serine/threonine phosphatases and aquaporin-2 regulation in renal collecting duct. Am J Physiol Renal Physiol 312:F84-F95
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Chen, Lihe; Lee, Jae Wook; Chou, Chung-Lin et al. (2017) Transcriptomes of major renal collecting duct cell types in mouse identified by single-cell RNA-seq. Proc Natl Acad Sci U S A 114:E9989-E9998
Zhao, Yue; Yang, Chin-Rang; Raghuram, Viswanathan et al. (2016) BIG: A large-scale data integration tool for renal physiology. Am J Physiol Renal Physiol :ajprenal.00249.2016
Cheng, Lei; Pisitkun, Trairak; Knepper, Mark A et al. (2016) Peptide Labeling Using Isobaric Tagging Reagents for Quantitative Phosphoproteomics. Methods Mol Biol 1355:53-70
Pickering, Christina M; Grady, Cameron; Medvar, Barbara et al. (2016) Proteomic profiling of nuclear fractions from native renal inner medullary collecting duct cells. Physiol Genomics 48:154-66

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