To realize nano scale x-ray imaging on a benchtop scale without highly specialized and costly x-ray optical componenets, we are developing an amplified photoresist film that is sensitive to x-ray and electron beam radiation, and can support nanometer scale features. These features are read accurately quantified by a compact atomic force microscopy. We have achieved initial success in a gold amplified E-beam resist system and obtained radiographs of x-ray gratings at 50 nm resolution. Concurrently, we are developing a new way to observe the interaction of x-ray with biological samples by imaging the reactive oxygen species produced by the x-ray beam. This approach is aimed to improve the contrast and specificity of nanometer scale x-ray microscopy of biological samples.

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National Heart, Lung, and Blood Institute
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Morales, A G; Stempinski, E S; Xiao, X et al. (2016) Micro-CT scouting for transmission electron microscopy of human tissue specimens. J Microsc 263:113-7
Gomella, Andrew; Martin, Eric W; Lynch, Susanna K et al. (2013) Low dose hard x-ray contact microscopy assisted by a photoelectric conversion layer. AIP Adv 3:42121