Sickle cell disease is the most common monogenic genetic disease in the United States. Mortality is higher in those with elevated pulmonary pressures by echocardiography or other markers of disease severity including frequent pain, iron overload and renal insufficiency. We have enrolled 795 subjects and 125 controls in a study of the prevalence and prognosis of subjects with sickle cell disease and suspected pulmonary hypertension. This represents approximately a 1% sample of patients with sickle cell disease living in the United States. All subjects are screened with transthoracic echocardiograms and the tricuspid regurgitant jet velocity (TRV) used to estimate the pulmonary artery systolic pressure. Additional phenotypic information related to the manifestations of sickle cell disease is also collected at evaluation. Suspected pulmonary hypertension was prospectively defined by a TRV >= 2.5 m/sec and severe pulmonary hypertension defined by a TRV >= 3.0 m/sec. Subjects have been followed for a mean of 6 years and censored at time of death or loss to follow-up. There were 41 new subjects enrolled at the NIH during this year; 5 were controls and 36 were subjects with sickle cell disease. There were no new subjects enrolled at Howard University. Total enrolled at Howard University is 131 and the total enrolled at NIH is 789. Total enrollment for all sites is 920. Subject enrollment is ongoing. We are conducting 2, 4, 6, 8, 10 and 12 year follow-up visits for comprehensive data collection. We also completed a comprehensive survival update on all subjects, the first since 2010. All new subjects had corresponding biospecimens collected for genomic investigations. Our goal is to recruit at least 1000 subjects with sickle cell disease for initial exploratory genetic studies. This will allow for sufficient statistical power to preliminarily identify genetic modifiers. Variants in the GCH1 gene, which have previously been identified as modulators of pain in other diseases, are associated with increased risk of severe vaso-occlusive pain crises in a case control study. We also replicated these findings in an independent cohort. GCH1 is the rate limiting step for the synthesis of BH4 (tetrahydrobiopterin) which is an essential co-factor for the synthesis of nitric oxide, catecholamines, serotonin, and phenylalanine metabolism. In vitro analysis of whole blood samples from subjects with sickle cell disease shows that this GCH1 haplotype is associated with higher BH4 levels. This result indicates that this is a unique African haplotype associated with increased BH4 and increased pain. This haplotype is also associated with altered vascular function during forearm blood flow studies in adults with sickle cell disease. Surprisingly, this haplotype is not associated with pulmonary hypertension in SCD despite the biological suggestion that the African haplotype might promote nitric oxide production. Variants in the MYH9 gene are associated with decreased glomerular filtration rate in SCD. Genetic markers in the region of MYH9 on chromosome 22 have been identified as major risk factors for focal segmental glomerular sclerosis (FSGS) in African American populations. This candidate gene association study extends this work to a disease specific patient population that is at high risk for renal insufficiency. At the same time, we are also using gene wide admixture markers to perform a genome wide association study for this phenotype by admixture mapping. These genome wide data will also allow for assessment of both local and global ancestry relative to the MYH9 locus. A genome wide association study several phenotypes in sickle cell anemia is in progress using ancestry informative markers that were identified from the HapMap project. We have designed a genome wide panel of 1796 ancestry informative markers and have completed genotyping in more than 1700 subjects with sickle cell disease and 2 HapMap study populations. Analysis of population structure using this panel demonstrates that a proportion of sickle cell disease patients living in the United States have very little genetic admixture. The degree of population admixture is also significantly different when comparing sickle cell disease populations from different geographic regions. These results suggest that genetic studies of sickle cell disease may require additional statistical adjustments when combining cohorts. The highly variable degree of admixture also implies that statistical power may be significantly lower than current predictions. For genome wide association studies, we will perform a combined analysis with samples from the Bethesda Sickle Cell Cohort Study (Clinicaltrials.gov Identifier NCT00011648) and the WalkPhasst clinical trial screening population (Clinicaltrials.gov Identifier NCT00492531). Initially, we will analyze pain and HbF as phenotypes. The phenotype defining cases in this study is >1 severe pain crisis per year which provides >80% statistical power to detect genome wide associations with a relative risk of 2 or higher. Over the past year, an admixture GWAS in the Bethesda Sickle Cell Cohort Study shows no loci associated with pain crisis events, while a scan using sleep disturbance defined by the Pittsburgh Sleep Quality Index is suggestive of linkage for markers on chromosome 13. Studies are also underway to also determine if gene expression profiling can also be used to identify sub-phenotypes of sickle cell disease. In a pilot study, we have isolated RNA from different peripheral blood preparations to determine which is the most informative for larger studies. For this pilot, subjects with SS sickle cell disease were compared to those with SC sickle cell disease because they each have distinct clinical manifestations. Preliminary analysis suggests that SS and SC have nearly identical gene expression profiles despite their clinical differences. In a larger follow-up study, we compared gene expression profiles for 2 sickle cell anemia sub-phenotypes defined by a plasma biomarker using exon arrays. We have identified candidate genes associated with this sub-phenotype, including several alternatively spliced transcripts. Finally, we have collected total blood RNA from a cohort of 30 sickle cell anemia subjects phenotyped for pain and prospectively followed for 12 months. MicroRNA profiles from 2 crisis events from the same subject are being compared to baseline profiles is a study designed to identify biomarkers for pain crisis events. Finally, a trial is underway to characterize quantitative sensory testing in adults with sickle cell anemia. Specifically, a mechanical temporal summation pain phenotype suggestive of centralized pain has been identified by our study of pain in sickle cell anemia. The temporal summation assay is specific for hyperexcitability at the synapse between the sensory afferent and the central nervous system. The sensory afferent cell body (dorsal nerve root ganglion) is the site where the GCH1 transcript was overexpressed in animal models of chronic pain. We hypothesize that the GCH1 gene regulates chronic pain through alteration of synaptic transmission from the sensory afferent to the posterior columns in the spinal cord. Therefore, temporal summation data is being collected from a larger sickle cell anemia population to determine if this centralized pain phenotype is associated with GCH1 variants.

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4
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2015
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U.S. National Heart Lung and Blood Inst
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Darbari, Deepika S; Vaughan, Kathleen J; Roskom, Katherine et al. (2017) Central sensitization associated with low fetal hemoglobin levels in adults with sickle cell anemia. Scand J Pain 17:279-286
Hosokawa, Kohei; Muranski, Pawel; Feng, Xingmin et al. (2016) Memory Stem T Cells in Autoimmune Disease: High Frequency of Circulating CD8+ Memory Stem Cells in Acquired Aplastic Anemia. J Immunol 196:1568-78
Webb, R Chad; Ma, Yinji; Krishnan, Siddharth et al. (2015) Epidermal devices for noninvasive, precise, and continuous mapping of macrovascular and microvascular blood flow. Sci Adv 1:e1500701
Hosokawa, Kohei; Muranski, Pawel; Feng, Xingmin et al. (2015) Identification of novel microRNA signatures linked to acquired aplastic anemia. Haematologica :
Darbari, Deepika S; Hampson, Johnson P; Ichesco, Eric et al. (2015) Frequency of Hospitalizations for Pain and Association With Altered Brain Network Connectivity in Sickle Cell Disease. J Pain 16:1077-86
Fitzhugh, Courtney D; Hsieh, Matthew M; Allen, Darlene et al. (2015) Hydroxyurea-Increased Fetal Hemoglobin Is Associated with Less Organ Damage and Longer Survival in Adults with Sickle Cell Anemia. PLoS One 10:e0141706
Feld, J J; Kato, G J; Koh, C et al. (2015) Liver injury is associated with mortality in sickle cell disease. Aliment Pharmacol Ther 42:912-21
van Beers, Eduard J; Yang, Yanqin; Raghavachari, Nalini et al. (2015) Iron, inflammation, and early death in adults with sickle cell disease. Circ Res 116:298-306
Wallen, Gwenyth R; Minniti, Caterina P; Krumlauf, Michael et al. (2014) Sleep disturbance, depression and pain in adults with sickle cell disease. BMC Psychiatry 14:207
Milton, Jacqueline N; Gordeuk, Victor R; Taylor 6th, James G et al. (2014) Prediction of fetal hemoglobin in sickle cell anemia using an ensemble of genetic risk prediction models. Circ Cardiovasc Genet 7:110-5

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