Dr. Spouge was invited to the Edinburgh Conference """"""""Selecting Barcode Loci for Plants"""""""", a gathering of a subset of the Plant Working Group within the Consortium for the Barcode of Life, to help select a plant barcode. The other conference participants sequenced 7 candidate barcode loci across a broad range of land plants, so Dr Spouge and Damon Little of the New York Botanical Garden could analyze the resulting data, to permit the conference participants to select markers for a plant barcode. The Edinburgh Conference selected rbcL and matK as the 2 plant barcode markers, from 7 choices. Dr. Spouge performed a similar data analysis for the Fungal Barcode Working Group in Amsterdam (2011), which selected ITS (the Internal Transcribed Spacer between structural ribosomal RNAs) from 4 choices. He offered similar services to the Protist Barcode Working Group in Berlin (2011), but insufficient data prevented a decisive analysis. He had made implementations of his analysis publicly available in user-friendly programs, to permit barcode researchers to carry out their own analyses. The analyses are likely to be particularly important in selecting secondary markers in different fungal clades.
|Suwannasai, Nuttika; Martin, Maria P; Phosri, Cherdchai et al. (2013) Fungi in Thailand: a case study of the efficacy of an ITS barcode for automatically identifying species within the Annulohypoxylon and Hypoxylon genera. PLoS One 8:e54529|
|Pawlowski, Jan; Audic, Stephane; Adl, Sina et al. (2012) CBOL protist working group: barcoding eukaryotic richness beyond the animal, plant, and fungal kingdoms. PLoS Biol 10:e1001419|
|Schoch, Conrad L; Seifert, Keith A; Huhndorf, Sabine et al. (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proc Natl Acad Sci U S A 109:6241-6|
|Spouge, John L; Mariño-Ramírez, Leonardo (2012) The practical evaluation of DNA barcode efficacy. Methods Mol Biol 858:365-77|
|CBOL Plant Working Group (2009) A DNA barcode for land plants. Proc Natl Acad Sci U S A 106:12794-7|