Further work on the cellular mechanisms underlying neurologic and pscyhiatric disorders is limited by the fact that neurons throughout the brain utilize common mechanisms for critical functions such as synaptic plasticity, whereas specific neural systems are affected in different disorders. A large part of the Laboratory is now working with the Gene Expression Nervous System Atlas (GENSAT) project ( funded by NINDS and NIMH ) to produce transgenic mice with Cre-recombinase under the regulation of promoters to drive expression in specific neuron populations. Our part of this work is to characterize the expression of Cre in the brains of such transgenic lines and to provide such lines to the Mutant Mouse Regional Resource Center (MMRRC) for distribution to the research community ( Gong et al., 2007). In the past year we characterized 200 Cre driver lines of which 60 were added to those made available through the GENSAT project: www.gensat.org/index.html .

Project Start
Project End
Budget Start
Budget End
Support Year
23
Fiscal Year
2012
Total Cost
$1,705,193
Indirect Cost
Name
U.S. National Institute of Mental Health
Department
Type
DUNS #
City
State
Country
Zip Code
Economo, Michael N; Clack, Nathan G; Lavis, Luke D et al. (2016) A platform for brain-wide imaging and reconstruction of individual neurons. Elife 5:e10566
De Bundel, D; Zussy, C; Espallergues, J et al. (2016) Dopamine D2 receptors gate generalization of conditioned threat responses through mTORC1 signaling in the extended amygdala. Mol Psychiatry 21:1545-1553
Gerfen, Charles R; Sawchenko, Paul E (2016) An anterograde neuroanatomical tracing method that shows the detailed morphology of neurons, their axons and terminals: Immunohistochemical localization of an axonally transported plant lectin, Phaseolus vulgaris-leucoagglutinin (PHA-L). Brain Res 1645:42-5
Viswanathan, Sarada; Williams, Megan E; Bloss, Erik B et al. (2015) High-performance probes for light and electron microscopy. Nat Methods 12:568-76
Li, Nuo; Chen, Tsai-Wen; Guo, Zengcai V et al. (2015) A motor cortex circuit for motor planning and movement. Nature 519:51-6
Saunders, Arpiar; Oldenburg, Ian A; Berezovskii, Vladimir K et al. (2015) A direct GABAergic output from the basal ganglia to frontal cortex. Nature 521:85-9
Paletzki, Ronald; Gerfen, Charles R (2015) Whole Mouse Brain Image Reconstruction from Serial Coronal Sections Using FIJI (ImageJ). Curr Protoc Neurosci 73:1.25.1-21
Mastwal, Surjeet; Ye, Yizhou; Ren, Ming et al. (2014) Phasic dopamine neuron activity elicits unique mesofrontal plasticity in adolescence. J Neurosci 34:9484-96
Oh, Seung Wook; Harris, Julie A; Ng, Lydia et al. (2014) A mesoscale connectome of the mouse brain. Nature 508:207-14
Gangarossa, Giuseppe; Espallergues, Julie; de Kerchove d'Exaerde, Alban et al. (2013) Distribution and compartmental organization of GABAergic medium-sized spiny neurons in the mouse nucleus accumbens. Front Neural Circuits 7:22

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