As part of an integrated effort between the Pathogenetics Unit and the Urologic Oncology Branch, CCR, we identified a large number of deregulated transcripts and proteins in human prostate cancer, including a gene set that segregates high- and moderate grade cancer, and a gene set that segregates androgen-dependent and androgen-independent disease. Efforts are continuing to better understand the molecular nature of prostate tumorigenesis, and to identify gene expression profiles associated with clinically aggressive cancer. Additionally, we discovered a new type of epigenetic methylation field in prostate cancer that may assist in understanding the pathogenesis of the tumor microenvironment. In a search for genes involved in the development of esophageal tumors, we are collaborating with the Cancer Prevention Studies Branch, CCR, to examine the molecular basis of esophageal squamous cell carcinoma (ESCC) in a high-risk population in Shanxi province in China. Allelotype comparison of patients with and without a family history of ESCC identified a region on chromosome band 13q12 that may harbor a familial tumor suppressor gene. Efforts are underway to analyze several candidate genes in the genomic region of interest. Multiple Endocrine Neoplasia Type I (MEN1) is an inherited syndrome characterized by development of neuroendocrine tumors in affected individuals. The responsible gene was discovered in 1997 by the NIH MEN1 working group, including the Pathogenetics Unit and groups from the National Human Genome Research Institute, the National Center for Biotechnology Information, and the National Institute of Diabetes and Digestive and Kidney Diseases. Current efforts of the Pathogenetics Unit include immunohistochemical evaluation of menin in human tissues.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIASC010437-13
Application #
8763716
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
2013
Total Cost
$562,873
Indirect Cost
Name
National Cancer Institute Division of Clinical Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Rodriguez-Canales, Jaime; Hanson, Jeffrey C; Hipp, Jason D et al. (2013) Optimal molecular profiling of tissue and tissue components: defining the best processing and microdissection methods for biomedical applications. Methods Mol Biol 980:61-120
Yan, Wusheng; Shih, Joanna; Rodriguez-Canales, Jaime et al. (2013) Three-dimensional mRNA measurements reveal minimal regional heterogeneity in esophageal squamous cell carcinoma. Am J Pathol 182:529-39
Yan, Wusheng; Shih, Joanna H; Rodriguez-Canales, Jaime et al. (2012) Identification of unique expression signatures and therapeutic targets in esophageal squamous cell carcinoma. BMC Res Notes 5:73
Roy Chowdhuri, Sinchita; Hanson, Jeffrey; Cheng, Jerome et al. (2012) Semiautomated laser capture microdissection of lung adenocarcinoma cytology samples. Acta Cytol 56:622-31
Chowdhuri, Sinchita Roy; Xi, Liqiang; Pham, Trinh Hoc-Tran et al. (2012) EGFR and KRAS mutation analysis in cytologic samples of lung adenocarcinoma enabled by laser capture microdissection. Mod Pathol 25:548-55
Gannot, Gallya; Richardson, Annely M; Rodriguez-Canales, Jaime et al. (2011) Decrease in CD8+ lymphocyte number and altered cytokine profile in human prostate cancer. Am J Cancer Res 1:120-127
Rodriguez-Canales, Jaime; Eberle, Franziska C; Jaffe, Elaine S et al. (2011) Why is it crucial to reintegrate pathology into cancer research? Bioessays 33:490-8
Yan, Wusheng; Wistuba, Ignacio I; Emmert-Buck, Michael R et al. (2011) Squamous Cell Carcinoma - Similarities and Differences among Anatomical Sites. Am J Cancer Res 1:275-300
Zhu, Liang; Yan, Wusheng; Rodriguez-Canales, Jaime et al. (2011) MicroRNA analysis of microdissected normal squamous esophageal epithelium and tumor cells. Am J Cancer Res 1:574-584
Erickson, Heidi S; Gannot, Gallya; Tangrea, Michael A et al. (2010) High throughput screening of normal and neoplastic tissue samples. Comb Chem High Throughput Screen 13:253-67

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