The Vaccine Research Center Flow Cytometry Core provides is a service facility providing support for the basic research and clinical program of the VRC. The Core maintains five 18-color analyzers and 2 18-color sorters, of which one is in a BSL-3 facility. The core performs routine Quality Assurance and Quality Control for all instruments, ensuring consistent, robust performance. The Core also provides direct support to researchers at the VRC at multiple levels: designing flow cytometry experiments, implementing the experiments, and analysis of the data that results. In some cases, the core provides support by running the samples for the users on the instruments. The Core is responsible for interactions with the instrument manufacturer to schedule all routine and unexpected maintenance and service requests. Finally, the Core provides routine and advanced training to users for instrument use and data analysis.

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6
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2014
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Fletez-Brant, Kipper; Å pidlen, Josef; Brinkman, Ryan R et al. (2016) flowClean: Automated identification and removal of fluorescence anomalies in flow cytometry data. Cytometry A 89:461-71
Ishizuka, Andrew S; Lyke, Kirsten E; DeZure, Adam et al. (2016) Corrigendum: Protection against malaria at 1 year and immune correlates following PfSPZ vaccination. Nat Med 22:692
Ishizuka, Andrew S; Lyke, Kirsten E; DeZure, Adam et al. (2016) Protection against malaria at 1 year and immune correlates following PfSPZ vaccination. Nat Med 22:614-23
Roederer, Mario (2016) Distributions of autofluorescence after compensation: Be panglossian, fret not. Cytometry A 89:398-402
Tapia, Milagritos D; Sow, Samba O; Lyke, Kirsten E et al. (2016) Use of ChAd3-EBO-Z Ebola virus vaccine in Malian and US adults, and boosting of Malian adults with MVA-BN-Filo: a phase 1, single-blind, randomised trial, a phase 1b, open-label and double-blind, dose-escalation trial, and a nested, randomised, double-bli Lancet Infect Dis 16:31-42
Robinson, J Paul; Roederer, Mario (2015) HISTORY OF SCIENCE. Flow cytometry strikes gold. Science 350:739-40
Roederer, Mario (2015) A proposal for unified flow cytometer parameter naming. Cytometry A 87:689-91
Perfetto, Stephen P; Chattopadhyay, Pratip K; Wood, James et al. (2014) Q and B values are critical measurements required for inter-instrument standardization and development of multicolor flow cytometry staining panels. Cytometry A 85:1037-48
Nguyen, Richard; Perfetto, Stephen; Mahnke, Yolanda D et al. (2013) Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design. Cytometry A 83:306-15
Britten, C M; Janetzki, S; Butterfield, L H et al. (2012) T cell assays and MIATA: the essential minimum for maximum impact. Immunity 37:1-2

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