Abstract

The facility has two objectives. First, its mission is to provide state-of-the-art light microscopy instrumentation for the Lab of Receptor Biology and Gene Expression. Second, the facility provides support to anyone in the CCR who requires assistance with fluorescence imaging. Present work in the facility revolves around four different general techniques: structural studies such as colocalization of different fluorescent markers, time-lapse analysis to follow how cellular components change over time, fluorescence recovery after photobleaching to measure mobilities and binding of cell molecues and fluorescence resonance energy transfer to measure molecular interactions in live or fixed specimens.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICBC010571-08
Application #
8350100
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
2011
Total Cost
$300,152
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Choy, John S; O'Toole, Eileen; Schuster, Breanna M et al. (2013) Genome-wide haploinsufficiency screen reveals a novel role for ?-TuSC in spindle organization and genome stability. Mol Biol Cell 24:2753-63
Case, Chanelle M; Sackett, Dan L; Wangsa, Danny et al. (2013) CKAP2 ensures chromosomal stability by maintaining the integrity of microtubule nucleation sites. PLoS One 8:e64575
Mazza, Davide; Abernathy, Alice; Golob, Nicole et al. (2012) A benchmark for chromatin binding measurements in live cells. Nucleic Acids Res 40:e119
Mazza, Davide; Stasevich, Timothy J; Karpova, Tatiana S et al. (2012) Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy. Methods Mol Biol 833:177-200
Mueller, Florian; Morisaki, Tatsuya; Mazza, Davide et al. (2012) Minimizing the impact of photoswitching of fluorescent proteins on FRAP analysis. Biophys J 102:1656-65
Mueller, Florian; Karpova, Tatiana S; Mazza, Davide et al. (2012) Monitoring dynamic binding of chromatin proteins in vivo by fluorescence recovery after photobleaching. Methods Mol Biol 833:153-76
Gordon, Shari N; Cecchinato, Valentina; Andresen, Vibeke et al. (2011) Smallpox vaccine safety is dependent on T cells and not B cells. J Infect Dis 203:1043-53
Chernova, Tatiana A; Romanyuk, Andrey V; Karpova, Tatiana S et al. (2011) Prion induction by the short-lived, stress-induced protein Lsb2 is regulated by ubiquitination and association with the actin cytoskeleton. Mol Cell 43:242-52
Heyerdahl, Sarah L; Rozenberg, Julian; Jamtgaard, Louis et al. (2010) The arylstibonic acid compound NSC13746 disrupts B-ZIP binding to DNA in living cells. Eur J Cell Biol 89:564-73
Rochman, Mark; Postnikov, Yuri; Correll, Sarah et al. (2009) The interaction of NSBP1/HMGN5 with nucleosomes in euchromatin counteracts linker histone-mediated chromatin compaction and modulates transcription. Mol Cell 35:642-56

Showing the most recent 10 out of 13 publications