The facility has two objectives. First, its mission is to provide state-of-the-art light microscopy instrumentation for the Lab of Receptor Biology and Gene Expression. Second, the facility provides support to anyone in the CCR who requires assistance with fluorescence imaging. Present work in the facility revolves around four different general techniques: structural studies such as colocalization of different fluorescent markers, time-lapse analysis to follow how cellular components change over time, fluorescence recovery after photobleaching to measure mobilities and binding of cell molecues and fluorescence resonance energy transfer to measure molecular interactions in live or fixed specimens.
|Case, Chanelle M; Sackett, Dan L; Wangsa, Danny et al. (2013) CKAP2 ensures chromosomal stability by maintaining the integrity of microtubule nucleation sites. PLoS One 8:e64575|
|Sung, Myong-Hee; Bagain, Lorena; Chen, Zhong et al. (2008) Dynamic effect of bortezomib on nuclear factor-kappaB activity and gene expression in tumor cells. Mol Pharmacol 74:1215-22|
|Nishiyama, Akira; Mochizuki, Kazuki; Mueller, Florian et al. (2008) Intracellular delivery of acetyl-histone peptides inhibits native bromodomain-chromatin interactions and impairs mitotic progression. FEBS Lett 582:1501-7|
|Catalfamo, Marta; Tai, Xuguang; Karpova, Tatiana et al. (2008) TcR-induced regulated secretion leads to surface expression of CTLA-4 in CD4+CD25+ T cells. Immunology 125:70-9|