The major activity of the core is to produce high quality microarrays. The equipment available at the core includes a fully functional tissue culture laboratory, niGrid Arrayer, GeneMachines, Biomek FX Laboratory Automation Workstation, DNA Engine Tetrad Thermal Cycler (2 units), SpeedVac Concentrator, Super T 21 Refrigerated Centrifuge, Ultrospec UV/Visible Spectrophotometer, GenePix 4000 Array Scanner (2 units), DNA Engine Opticon 2 Real-Time PCR Detection System, Z2 Coulter Counter, Beckman Coulter, and access to other equipment available at the Radiation Oncology Branch. The annual production of microarrays for ROB scientists is approximately 1500. Other investigators in the printing of microarrays is as follows, Dr. L. Staudt, CCR (1000).

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICBC010991-02
Application #
7969998
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2009
Total Cost
$681,343
Indirect Cost
Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Wahba, Amy; Ryan, Michael C; Shankavaram, Uma T et al. (2018) Radiation-induced alternative transcripts as detected in total and polysome-bound mRNA. Oncotarget 9:691-705
Sproull, Mary; Kramp, Tamalee; Tandle, Anita et al. (2017) Multivariate Analysis of Radiation Responsive Proteins to Predict Radiation Exposure in Total-Body Irradiation and Partial-Body Irradiation Models. Radiat Res 187:251-258
Celiku, Orieta; Tandle, Anita; Chung, Joon-Yong et al. (2017) Computational analysis of the mesenchymal signature landscape in gliomas. BMC Med Genomics 10:13