We have used conventional chemical fixation, dehydration, embedding and staining procedures to carry out studies on several fundamental biological processes, including membrane fusion, macroautophagy, and motility. We established a number of collaborative projects with multiple institutes across NIH. For example, with NIA we performed studies on the neuroprotective mechanism of NAM, the NAD+ precursor, and its effects on the autophagy-system in neurons exposed to oxidative stress;with NIDCR we examined mucin-type O-linked glycosylation in the structures of digestive system during development in Drosophila;and with NHLBI we investigated the process of selective degradation of mitochondria by using Drosophila cell lines. We also collaborated with a group in The Johns Hopkins University School of Medicine to use a high-pressure freezing/freeze-substitution method, the most useful and powerful cryofixation method, to study dynamic rearrangements of the actin cytoskeleton in Drosophila. The findings were published in the May issue of Developmental Cell. In addition, quite a few projects involving the characterization of nanoparticles and drug delivery mechanisms were performed by using electron microscopy techniques, including negative staining methods. Investigators within the IRPs of NIBIB, NIDDK and NINDS initiated most of these projects.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
National Institute of Biomedical Imaging and Bioengineering
Zip Code
Bryant Jr, L Henry; Kim, Saejeong J; Hobson, Matthew et al. (2016) Physicochemical Characterization of Ferumoxytol, Heparin and Protamine Nanocomplexes for improved magnetic labeling of stem cells. Nanomedicine :
Lin, Jing; Wang, Min; Hu, Hao et al. (2016) Multimodal-Imaging-Guided Cancer Phototherapy by Versatile Biomimetic Theranostics with UV and γ-Irradiation Protection. Adv Mater 28:3273-9
Zhang, Fan; Qi, Yun; Zhou, Kiet et al. (2015) The cAMP phosphodiesterase Prune localizes to the mitochondrial matrix and promotes mtDNA replication by stabilizing TFAM. EMBO Rep 16:520-7
Wang, Zhe; Wang, Yu; Wang, Zhiyong et al. (2015) Polymeric Nanovehicle Regulated Spatiotemporal Real-Time Imaging of the Differentiation Dynamics of Transplanted Neural Stem Cells after Traumatic Brain Injury. ACS Nano 9:6683-95
Huang, Peng; Gao, Yuan; Lin, Jing et al. (2015) Tumor-Specific Formation of Enzyme-Instructed Supramolecular Self-Assemblies as Cancer Theranostics. ACS Nano :
Shomorony, A; Pfeifer, C R; Aronova, M A et al. (2015) Combining quantitative 2D and 3D image analysis in the serial block face SEM: application to secretory organelles of pancreatic islet cells. J Microsc 259:155-64
Qi, Y; Liu, H; Daniels, M P et al. (2015) Loss of Drosophila i-AAA protease, dYME1L, causes abnormal mitochondria and apoptotic degeneration. Cell Death Differ :
Chen, Richard J; Zhang, Guofeng; Garfield, Susan H et al. (2015) Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States. PLoS One 10:e0142554
Kim, Ji Hoon; Ren, Yixin; Ng, Win Pin et al. (2015) Mechanical tension drives cell membrane fusion. Dev Cell 32:561-73
Pfeifer, C R; Shomorony, A; Aronova, M A et al. (2015) Quantitative analysis of mouse pancreatic islet architecture by serial block-face SEM. J Struct Biol 189:44-52

Showing the most recent 10 out of 30 publications