Our purpose is provided state of the art fluorescence activated cell sorting technology to the investigators at NIEHS including assistance on experimental design, instrument operation, and data analysis. The lab is equipped with 7 diverse and uniquely customized instruments and 1 workstations with various flow cytometric computer programs for off line and in depth data analysis, including presentation/multi-color analysis software. Currently there are 160 individual scientists at NIEHS who are trained and have access to these instruments. Over the past year, we have accomplished 1,447 projects, including 214 physical sorts where cells were isolated for further culture, injection, or biochemical analysis. The scope of these projects cover a broad range of scientific investigation including viability assessment, fluorescent protein expression, cell size and intracellular ion analysis, immunophenotyping, cell cycle profiling, and assessment of cell death. Additionally, several specialized studies involving fluorescent resonance energy transfer (FRET), intracellular organelle function, oxidative/phosphorylation, and calcium flux studies have been accomplished. Furthermore we have incorporate translational studies using samples obtained from the Clinical Research Unit. We provide a dedicated instrument and expertise to examine the effects of numerous environmental agents using human derived samples to better understand the relationship between us and our surroundings.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Zip Code
Joo, Joung Hyuck; Ueda, Eiichiro; Bortner, Carl D et al. (2015) Farnesol activates the intrinsic pathway of apoptosis and the ATF4-ATF3-CHOP cascade of ER stress in human T lymphoblastic leukemia Molt4 cells. Biochem Pharmacol 97:256-68
Yong, Sheila T; Nguyen, Hoai-Nghia; Choi, Jae H et al. (2015) Identification of a functional nuclear translocation sequence in hPPIP5K2. BMC Cell Biol 16:17
Nakano, Hideki; Moran, Timothy P; Nakano, Keiko et al. (2015) Complement receptor C5aR1/CD88 and dipeptidyl peptidase-4/CD26 define distinct hematopoietic lineages of dendritic cells. J Immunol 194:3808-19
Stober, Vandy P; Szczesniak, Christopher; Childress, Quiana et al. (2014) Bronchial epithelial injury in the context of alloimmunity promotes lymphocytic bronchiolitis through hyaluronan expression. Am J Physiol Lung Cell Mol Physiol 306:L1045-55
Brown, Paula R; Odet, Fanny; Bortner, Carl D et al. (2014) Reporter mice express green fluorescent protein at initiation of meiosis in spermatocytes. Genesis 52:976-84
Bortner, Carl D; Cidlowski, John A (2014) Ion channels and apoptosis in cancer. Philos Trans R Soc Lond B Biol Sci 369:20130104
Franco, Rodrigo; Bortner, Carl D; Schmitz, Ingo et al. (2014) Glutathione depletion regulates both extrinsic and intrinsic apoptotic signaling cascades independent from multidrug resistance protein 1. Apoptosis 19:117-34
Kang, Hong Soon; Liao, Grace; DeGraff, Laura M et al. (2013) CD44 plays a critical role in regulating diet-induced adipose inflammation, hepatic steatosis, and insulin resistance. PLoS One 8:e58417
Nakano, H; Burgents, J E; Nakano, K et al. (2013) Migratory properties of pulmonary dendritic cells are determined by their developmental lineage. Mucosal Immunol 6:678-91
Li, Hong; Edin, Matthew L; Bradbury, J Alyce et al. (2013) Cyclooxygenase-2 inhibits T helper cell type 9 differentiation during allergic lung inflammation via down-regulation of IL-17RB. Am J Respir Crit Care Med 187:812-22

Showing the most recent 10 out of 25 publications