The DNA Sequencing Core Lab provides a variety of services to the NIEHS scientific community. The Sequencing Core assist labs in SNP discovery, mutagenesis detection, custom primer design, expression construct design, and optimization of PCR. Our main focus has been to provide DNA sequence data to individual investigators in a timely manner. The DNA Sequencing Core works with individual labs to make DNA expression constructs. In the past year we have made DNA expression constructs involved in cell migration, cell adhesion, cancer metastasis, cancer related hormones, DNA repair excision repair mechanisms and for the fluorescent labeling of cells. The various constructs were used to study a host of cancers including but not limited to breast cancer, prostrate cancer, liver cancer, and brain cancer. The Sequencing Core has also collaborates with investigators at NCI to further understand the SPANX gene family. Genetic linkage studies indicate that germ line variations in a gene or genes on chromosome Xq27-28 are implicated in prostate carcinogenesis. The linkage peak of prostate cancer overlies a region of approximately 750 kb containing five SPANX genes (SPANX-A1, -A2, -B, -C, and -D) encoding sperm proteins associated with the nucleus;their expression was also detected in a variety of cancers. We continue to further characterize this region of the genome. We speculate that the predisposition to prostate cancer in X-linked families is an example of a genomic disease caused by a specific architecture of the SPANX gene cluster. Currently we are investigating the possibility of two new gens located in this region of the X chromosome. One a new SPANX family member and the other a micro RNA encoding gene.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Zip Code
Yin, Zhengyu; Menendez, Daniel; Resnick, Michael A et al. (2012) RAP80 is critical in maintaining genomic stability and suppressing tumor development. Cancer Res 72:5080-90
Zhang, Shu-Yun; Surapureddi, Sailesh; Coulter, Sherry et al. (2012) Human CYP2C8 is post-transcriptionally regulated by microRNAs 103 and 107 in human liver. Mol Pharmacol 82:529-40
Jiang, Chuancang; Zhao, Ming-Lang; Waters, Katherine M et al. (2012) Activation-induced deaminase contributes to the antibody-independent role of B cells in the development of autoimmunity. Autoimmunity 45:440-8
Lee, Su-Jun; Goldstein, Joyce A (2012) Comparison of CYP3A4 and CYP3A5: the effects of cytochrome b5 and NADPH-cytochrome P450 reductase on testosterone hydroxylation activities. Drug Metab Pharmacokinet 27:663-7
Kim, Yong-Sik; Kang, Hong Soon; Takeda, Yukimasa et al. (2012) Glis3 regulates neurogenin 3 expression in pancreatic β-cells and interacts with its activator, Hnf6. Mol Cells 34:193-200
Takeda, Yukimasa; Jothi, Raja; Birault, Veronique et al. (2012) RORγ directly regulates the circadian expression of clock genes and downstream targets in vivo. Nucleic Acids Res 40:8519-35
Sobhany, Mack; Kakuta, Yoshimitsu; Sugiura, Nobuo et al. (2012) The structural basis for a coordinated reaction catalyzed by a bifunctional glycosyltransferase in chondroitin biosynthesis. J Biol Chem 287:36022-8
Kirby, Thomas W; DeRose, Eugene F; Cavanaugh, Nisha A et al. (2012) Metal-induced DNA translocation leads to DNA polymerase conformational activation. Nucleic Acids Res 40:2974-83
García-Villada, Libertad; Drake, John W (2012) The three faces of riboviral spontaneous mutation: spectrum, mode of genome replication, and mutation rate. PLoS Genet 8:e1002832
Sam, Wai-Johnn; Chamberlain, Christine E; Lee, Su-Jun et al. (2011) Associations of ABCB1 3435C>T and IL-10-1082G>A polymorphisms with long-term sirolimus dose requirements in renal transplant patients. Transplantation 92:1342-7

Showing the most recent 10 out of 12 publications