During past year, the core continues to provide service and support to the NEI and NIH scientific community and to extramural researches on non-invasive methods to evalute visual function in various aniaml models. Visual Function Core also acquired a new Heidelberg Spectralis HRA+OCT system for fundus and OCT imaging of animal eyes. Demands for core to provide service on recordings full-field dark-adapted and light-adapted flash ERG and OCT retinal imaging remained strong. Core helped each user in design ERG recording and OCT imaging protocols tailored for specific needs. In addition, in collaboration with researchers, the core has developed a number of new approaches to evaluate visual function in various animal models, which include: 1) DC-ERG recording techniques to evaluate light elicited electrical responses mediated by RPE cells in the eye. 2) Photopic ERG elicited UV and green light for evaluate S and M cone function. 3) Visual-evoked potential recording techniques to non-invasively evaluate light-induced cortical responses. 4) OCT imaging of rabbit, squirrel, and rat eyes. 5) Using OptoMotry system to evaluate mouse vision (spatial and contrast sensitivity). VFC provided training and help to users on analysis methods of ERG and OCT image data, and on tools of generating final reports. Core also provided directions and instructions on installation of software needed for ERG and OCT data analysis. Core organized seminars and lectures on using ERG recording and OCT techniques for visual function evaluation. In addition, the core also hosted ERG workshop sessions to educate users on retinal physiology and techniques of ERG recording and data analysis. To advocate the service to NIH research community, the core presented a poster at NIH research festival. In addition, core provided consultations and advises on various research projects. During the past year, core provided service and training to 40 scientists in 20 research groups (PIs), including 2 from other NIH institutes and 1 extramural.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICEY000503-02
Application #
8557113
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2012
Total Cost
$175,963
Indirect Cost
Name
U.S. National Eye Institute
Department
Type
DUNS #
City
State
Country
Zip Code
Veleri, Shobi; Nellissery, Jacob; Mishra, Bibhudatta et al. (2017) REEP6 mediates trafficking of a subset of Clathrin-coated vesicles and is critical for rod photoreceptor function and survival. Hum Mol Genet 26:2218-2230
Fan, Jianguo; Jia, Li; Li, Yan et al. (2017) Maturation arrest in early postnatal sensory receptors by deletion of the miR-183/96/182 cluster in mouse. Proc Natl Acad Sci U S A 114:E4271-E4280
Wang, Xu; Zhao, Lian; Zhang, Yikui et al. (2017) Tamoxifen Provides Structural and Functional Rescue in Murine Models of Photoreceptor Degeneration. J Neurosci 37:3294-3310
Hinshaw, Samuel J H; Ogbeifun, Osato; Wandu, Wambui S et al. (2016) Digoxin Inhibits Induction of Experimental Autoimmune Uveitis in Mice, but Causes Severe Retinal Degeneration. Invest Ophthalmol Vis Sci 57:1441-7
Sun, Xun; Park, James H; Gumerson, Jessica et al. (2016) Loss of RPGR glutamylation underlies the pathogenic mechanism of retinal dystrophy caused by TTLL5 mutations. Proc Natl Acad Sci U S A 113:E2925-34
Li, Yan; Yu, Shirley; Duncan, Todd et al. (2015) Mouse model of human RPE65 P25L hypomorph resembles wild type under normal light rearing but is fully resistant to acute light damage. Hum Mol Genet 24:4417-28
Tuo, Jingsheng; Wang, Yujuan; Cheng, Rui et al. (2015) Wnt signaling in age-related macular degeneration: human macular tissue and mouse model. J Transl Med 13:330
Chen, J; Qian, H; Horai, R et al. (2015) Mouse Models of Experimental Autoimmune Uveitis: Comparative Analysis of Adjuvant-Induced vs Spontaneous Models of Uveitis. Curr Mol Med 15:550-7
Zhao, Lian; Zabel, Matthew K; Wang, Xu et al. (2015) Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration. EMBO Mol Med 7:1179-97
Wu, Zhijian; Hiriyanna, Suja; Qian, Haohua et al. (2015) A long-term efficacy study of gene replacement therapy for RPGR-associated retinal degeneration. Hum Mol Genet 24:3956-70

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