The Biophysics Core Facility service to the NIH scientific community consists of three main categories: direct research support, including instrument use, collaboration on research projects and scientific consultations;development and optimization of measurement methods and user training. In the reporting period the total instrument workload by NHLBI and NIH researchers in the Biophysics Core reached 3,012 hours. During this time, the Biophysics Core Facility users included principal investigators, staff scientists, postdocs and students from the following labs: NHLBI laboratories (listed alphabetically by the PI name): R. Balaban, J. Chung, A. Ferre-D'Amare, J. Hammer, E. Korn, R. Kotin, J. Lee, R. Levine, K. Neuman, A. Peterkofsky, A. Roll-Mecak, J. Sellers, J. Taraska, N. Tjandra, K. Zhao. Other NIH laboratories: R. Woodgate, (NICHD), C. Barry, J. Patton (both NIAID), P. Becerra (NEI), S. Buchanan (NIDDK), K. Gawrisch (NIAAA). The educational and training efforts of the Biophysics Core Facility led to the development of a series of introductory lectures covering details of specific techniques offered in the Core, and an overview presentation, which concisely describes all available techniques with examples of their applications. The Biophysics Core Facility co-organized the Workshop on Hydrodynamic and Thermodynamic Analysis of Macromolecules with SEDFIT and SEDPHAT. This workshop was conducted in May 2013 to teach strategies for hydrodynamic and thermodynamic analysis of sedimentation equilibrium (SE) and sedimentation velocity (SV) AUC, DLS, ITC and fluorescence spectroscopy for the characterization of biological macromolecules in solution. It consisted of over 35 hours of lectures and hands-on data analysis sessions. This workshop was attended not only by DIR researchers, but also by scientists from outside the NIH, with 27 registered trainees and over 50 researchers attending workshop lectures. Biophysics Core Facility users are also individually trained on particular techniques and on the instrument operation. Core Facility users request training online through the iLab system. In the reporting period the Biophysics Core Facility got 107 iLab requests.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
National Heart, Lung, and Blood Institute
Zip Code
Liu, W; Li, H; Hong, S-H et al. (2016) Olfactomedin 4 deletion induces colon adenocarcinoma in Apc(Min/+) mice. Oncogene :
Paez-Segala, Maria G; Sun, Mei G; Shtengel, Gleb et al. (2015) Fixation-resistant photoactivatable fluorescent proteins for CLEM. Nat Methods 12:215-8, 4 p following 218
Zhao, Huaying; Piszczek, Grzegorz; Schuck, Peter (2015) SEDPHAT--a platform for global ITC analysis and global multi-method analysis of molecular interactions. Methods 76:137-48
Gruschus, James M; Jiang, Zhiping; Yap, Thai Leong et al. (2015) Dissociation of glucocerebrosidase dimer in solution by its co-factor, saposin C. Biochem Biophys Res Commun 457:561-6
Zhao, Huaying; Ghirlando, Rodolfo; Alfonso, Carlos et al. (2015) A multilaboratory comparison of calibration accuracy and the performance of external references in analytical ultracentrifugation. PLoS One 10:e0126420
Fujiwara, Ikuko; Remmert, Kirsten; Piszczek, Grzegorz et al. (2014) Capping protein regulatory cycle driven by CARMIL and V-1 may promote actin network assembly at protruding edges. Proc Natl Acad Sci U S A 111:E1970-9
Stevens, Linda A; Barbieri, Joseph T; Piszczek, Grzegorz et al. (2014) Nonenzymatic conversion of ADP-ribosylated arginines to ornithine alters the biological activities of human neutrophil peptide-1. J Immunol 193:6144-51
Ghirlando, Rodolfo; Zhao, Huaying; Balbo, Andrea et al. (2014) Measurement of the temperature of the resting rotor in analytical ultracentrifugation. Anal Biochem 458:37-9
Rout, Ashok K; Strub, Marie-Paule; Piszczek, Grzegorz et al. (2014) Structure of transmembrane domain of lysosome-associated membrane protein type 2a (LAMP-2A) reveals key features for substrate specificity in chaperone-mediated autophagy. J Biol Chem 289:35111-23
Yu, Xiaozhen; Strub, Marie-Paule; Barnard, Travis J et al. (2014) An engineered palette of metal ion quenchable fluorescent proteins. PLoS One 9:e95808

Showing the most recent 10 out of 28 publications