1. The main services of the Pathology Core are histology procedures, such as formalin-fixed tissue embedding, sectioning and histology staining. For this purpose, the Core receives samples in fixed or in fresh condition. These samples are processed and embedded in paraffin or OCT compound for either paraffin or frozen sections. The paraffin embedded samples can be cut routinely as 5 micron thick sections mounted on glass slides. The serial sections through an entire organ or embryo, or mixed with thick (20-100 microns) and thin (5-10 microns) step sections also can be cut upon request. Frozen sections are normally cut at a thickness of 8-10 microns. The Pathology core provides routine histology staining with Hematoxylin and Eosin according to Harris methods (Laboratory Methods in Histotechnology. Armed Forces Institute of Pathology, Edited by Prophet EB, Mills B, Arrington JB and Sobin LH, pp 56, American Registry of Pathology, Washington, DC, 1992). In addition, we also perform special histology staining, including PAS, Masson tri-chrome, Movat, Elastic Van Gieson and Oil-Red O stain. All these services are performed by the Core staff and finished in a timely manner. Most requests are completed within a two-week period, except urgent requests that can be arranged as a higher-priority request. In some cases, results can be returned to users the next day. Since the last report, the pathology core provided these services for a total of 222 requests by 47 customers from 25 PIs/ laboratories. We have processed a total of 2,740 tissue blocks and cut 30,302 histology slides, which are usually mounted with 2 or 3 tissue sections. Among them, there were 5,245 slides stained with Hematoxylin and Eosin for routine histology. In addition, we also processed 1,799 slides for other specific staining procedures as mentioned above. 2. Diagnostic pathology. For this service,the Core head is involved in discussion of experimental design, sample collection methods and final evaluations of the results. We provide histology products such as cut and stained histology slides, perform immune-staining procedures, evaluate the results, describe the findings and make a scoring sheet with representative photographs. In most cases, this service will contribute to a manuscript submission and we will provide the final microscopic figures. We performed these services for 12 scientists from 6 PI/laboratories including histo-pathological diagnosis, histology evaluations with the score system and provided final images for publications. This resulted in 5 publications in 2012 and 4 in 2013. (See list of publications) 3. Training, consultation and trouble shooting for DIR scientists and fellows in the fields of histo-pathology and immuno-histochemistry. We provide training for tissue processing, embedding and sectioning for paraffin and frozen samples. In addition, we also offer testing for antibodies and for immuno-staining procedures and consultation for immuno-staining problems. We have trained 4 scientists/students for cutting frozen sections and 3 scientists for immunohistochemistry procedures. In the latter case,the Core head worked closely with the scientists for selection of antibodies and protocols, and then provided hands-on staining demonstration until the scientist can perform the procedures independently.

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National Heart, Lung, and Blood Institute
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Lin, Yongshun; Liu, Huimin; Klein, Michael et al. (2018) Efficient differentiation of cardiomyocytes and generation of calcium-sensor reporter lines from nonhuman primate iPSCs. Sci Rep 8:5907
Flores, Daniel J; Duong, ThuyVy; Brandenberger, Luke O et al. (2018) Conditional ablation and conditional rescue models for Casq2 elucidate the role of development and of cell-type specific expression of Casq2 in the CPVT2 phenotype. Hum Mol Genet 27:1533-1544
Baumer, Yvonne; Ng, Qimin; Sanda, Gregory E et al. (2018) Chronic skin inflammation accelerates macrophage cholesterol crystal formation and atherosclerosis. JCI Insight 3:
Wen, Han; Morales Martinez, Alejandro; Miao, Houxun et al. (2018) Correlative Detection of Isolated Single and Multi-Cellular Calcifications in the Internal Elastic Lamina of Human Coronary Artery Samples. Sci Rep 8:10978
Mishra, Amarjit; Yao, Xianglan; Saxena, Ankit et al. (2018) Low-density lipoprotein receptor-related protein 1 attenuates house dust mite-induced eosinophilic airway inflammation by suppressing dendritic cell-mediated adaptive immune responses. J Allergy Clin Immunol 142:1066-1079.e6
Xiong, Jianhua; Kawagishi, Hiroyuki; Yan, Ye et al. (2018) A Metabolic Basis for Endothelial-to-Mesenchymal Transition. Mol Cell 69:689-698.e7
Oh, Jangsuk; Wang, Yujuan; Chen, Shida et al. (2017) Genetic background-dependent role of Egr1 for eyelid development. Proc Natl Acad Sci U S A 114:E7131-E7139
West, Erin E; Spolski, Rosanne; Kazemian, Majid et al. (2016) A TSLP-complement axis mediates neutrophil killing of methicillin-resistant Staphylococcus aureus. Sci Immunol 1:
Liu, Julia C; Liu, Jie; Holmström, Kira M et al. (2016) MICU1 Serves as a Molecular Gatekeeper to Prevent In Vivo Mitochondrial Calcium Overload. Cell Rep 16:1561-1573
Yang, Zhi-Hong; Bando, Masahiro; Sakurai, Toshihiro et al. (2016) Long-chain monounsaturated fatty acid-rich fish oil attenuates the development of atherosclerosis in mouse models. Mol Nutr Food Res 60:2208-2218

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