The NHLBI Electron Microscopy Core Facility has supported projects using the following techniques in the past year: 1. Fixation, embedding, ultra-thin sectioning and transmission EM digital imaging of tissues and cells. 2. EM immunocytochemistry, including immunogold localization of proteins and other antigens within and on the surface of cells by pre-embedding and cryo-immunogold techniques. 3. Negative staining of large proteins, polymers and supramolecular structures as well as lipid and membrane vesicles for transmission EM imaging as well as platinum shadowing of large molecules. 4. Fixation, critical point drying, sputter-coating and scanning EM digital imaging of small organisms, organs, tissues and cells, as well as other materials such as artificial matrices. 5. Platinum carbon replicas of extracted, critical point dried cytoskeletons and the substrate-attached membranes of cultured cells In the past year, we have supported 25 projects for 18 principal investigators in the NHLBI/DIR and 1 project for a scientist outside the DIR. We have trained 10 postdoctoral fellows, students and contractors in the use of the scanning and transmission electron microscopes.

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National Heart, Lung, and Blood Institute
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Fertrin, Kleber Yotsumoto; van Beers, Eduard J; Samsel, Leigh et al. (2014) Imaging flow cytometry documents incomplete resistance of human sickle F-cells to ex vivo hypoxia-induced sickling. Blood 124:658-60
van Beers, Eduard J; Samsel, Leigh; Mendelsohn, Laurel et al. (2014) Imaging flow cytometry for automated detection of hypoxia-induced erythrocyte shape change in sickle cell disease. Am J Hematol 89:598-603
Liu, Xiong; Hong, Myoung-Soon; Shu, Shi et al. (2013) Regulation of the filament structure and assembly of Acanthamoeba myosin II by phosphorylation of serines in the heavy-chain nonhelical tailpiece. Proc Natl Acad Sci U S A 110:E33-40
Looze, Christopher; Yui, David; Leung, Lester et al. (2009) Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein. Biochem Biophys Res Commun 378:433-8
Liu, Jie; Cao, Liu; Chen, Jichun et al. (2009) Bmi1 regulates mitochondrial function and the DNA damage response pathway. Nature 459:387-92
McCroskery, S; Bailey, A; Lin, L et al. (2009) Transmembrane agrin regulates dendritic filopodia and synapse formation in mature hippocampal neuron cultures. Neuroscience 163:168-79
Cecchini, Sylvain; Negrete, Alejandro; Virag, Tamas et al. (2009) Evidence of prior exposure to human bocavirus as determined by a retrospective serological study of 404 serum samples from adults in the United States. Clin Vaccine Immunol 16:597-604
Smith, Richard H; Yang, Linda; Kotin, Robert M (2008) Chromatography-based purification of adeno-associated virus. Methods Mol Biol 434:37-54
Vergarajauregui, Silvia; Connelly, Patricia S; Daniels, Mathew P et al. (2008) Autophagic dysfunction in mucolipidosis type IV patients. Hum Mol Genet 17:2723-37
Greenberg, Cynthia C; Connelly, Patricia S; Daniels, Mathew P et al. (2008) Krp1 (Sarcosin) promotes lateral fusion of myofibril assembly intermediates in cultured mouse cardiomyocytes. Exp Cell Res 314:1177-91