In the past year, our core has assisted NHLBI investigators in successfully generating more than one dozen transgenic and knockout mouse lines. Besides these traditional services, the staffs in our core facility are also rigorously learning new skills and developing new capabilities, especially in embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) research areas. We have successfully derived many lines of mouse and human iPSCs, and become familiar with the methods for characterizing iPSC lines. By using the mouse blastocyst microinjection technique, we have assisted NHLBI scientists in examining the differentiation potential of ESCs and iPSCs into hematopoietic lineages in vivo. We have also devoted a considerable amount of effort in conducting gene-targeting experiments in human iPSCs using the zinc finger nuclease technology. Our goal is to generate several gene-targeted human iPSC reporter lines for facilitating cardiovascular differentiation. We have also been following the cutting edge methodologies for differentiating mouse and human iPSCs into beating cardiomyocytes. In addition, we have devoted a small portion of our effort on finishing up a research project on the characterization of a novel dwarf and obese mouse line generated in our facility using the gene-trapping method.

Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2011
Total Cost
$930,824
Indirect Cost
Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
Zip Code
Placek, Katarzyna; Hu, Gangqing; Cui, Kairong et al. (2017) MLL4 prepares the enhancer landscape for Foxp3 induction via chromatin looping. Nat Immunol 18:1035-1045
Lee, Hye Kyung; Willi, Michaela; Wang, Chaochen et al. (2017) Functional assessment of CTCF sites at cytokine-sensing mammary enhancers using CRISPR/Cas9 gene editing in mice. Nucleic Acids Res 45:4606-4618
Jang, Younghoon; Wang, Chaochen; Zhuang, Lenan et al. (2017) H3K4 Methyltransferase Activity Is Required for MLL4 Protein Stability. J Mol Biol 429:2046-2054
He, Yunlong; Zhu, Wentao; Shin, Min Hwa et al. (2017) cFOS-SOX9 Axis Reprograms Bone Marrow-Derived Mesenchymal Stem Cells into Chondroblastic Osteosarcoma. Stem Cell Reports 8:1630-1644
Shin, Ha Youn; Wang, Chaochen; Lee, Hye Kyung et al. (2017) CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome. Nat Commun 8:15464
Pan, Haihui; Yan, Ye; Liu, Chengyu et al. (2017) The role of ZKSCAN3 in the transcriptional regulation of autophagy. Autophagy 13:1235-1238
Sweeney, Colin L; Choi, Uimook; Liu, Chengyu et al. (2017) CRISPR-Mediated Knockout of Cybb in NSG Mice Establishes a Model of Chronic Granulomatous Disease for Human Stem-Cell Gene Therapy Transplants. Hum Gene Ther 28:565-575
Xu, Zhe; Xing, Shaojun; Shan, Qiang et al. (2017) Cutting Edge: ?-Catenin-Interacting Tcf1 Isoforms Are Essential for Thymocyte Survival but Dispensable for Thymic Maturation Transitions. J Immunol 198:3404-3409
Shin, Min Hwa; He, Yunlong; Marrogi, Eryney et al. (2016) A RUNX2-Mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells. PLoS Genet 12:e1005884
Liu, Julia C; Liu, Jie; Holmström, Kira M et al. (2016) MICU1 Serves as a Molecular Gatekeeper to Prevent In Vivo Mitochondrial Calcium Overload. Cell Rep 16:1561-1573

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