In the past year, our core has assisted NHLBI investigators in successfully generating more than one dozen transgenic and knockout mouse lines. One new capability our core has established during this year is the generation of targeted transgenic mouse lines, which is to insert a transgene into a specific genomic locus by co-injecting the transgene with the Phi31 recombinase into the pronuclei of fertilized eggs. We have also succeeded in deriving mouse embryonic stem cell (ESC) and epistem cell (EpiSC) lines from early mouse embryos. Our core has also devoted a substantial portion of our efforts on developing induced pluripotent stem cell (iPSC) related capabilities. We have gained a broad range of skills in this area, including iPSC derivation, random differentiation through embroid body formation and teratoma formation, karyotyping, and directed differentiation into cardiomyocyte and neuronal lineage. We have assisted several laboratories in deriving a number of mouse iPSC lines, and succeeded in deriving iPSC lines from a GFP-expressing transgenic pig. We have also succeeded in targeting reporter genes into a safe harbor locus (AAVS1) of human iPSCs derived from NIH patients. We aslo microinjected mouse iPSCs and hematopoietic stem cells (HSCs) into blastocysts collected from a knockout mouse strain, which is incapable of forming blood lineage. Throughout the year, we have provided numerous consultations and technical assistances to scientists with in and out of NHLBI to assist them in conducting mouse molecular genetic research, including designing DNA constructs, searching database, collecting various stages of embryos, deriving mouse embryonic fibroblasts (MEF), collecting mouse organs, and reconstituting mouse lines using cryopreserved sperm. I am also a member of the NHLBI Animal Care and Use Committee (ACUC) for reviewing animal protocols and conducting animal facility inspections.

Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
2012
Total Cost
$939,275
Indirect Cost
Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
Country
Zip Code
Placek, Katarzyna; Hu, Gangqing; Cui, Kairong et al. (2017) MLL4 prepares the enhancer landscape for Foxp3 induction via chromatin looping. Nat Immunol 18:1035-1045
Lee, Hye Kyung; Willi, Michaela; Wang, Chaochen et al. (2017) Functional assessment of CTCF sites at cytokine-sensing mammary enhancers using CRISPR/Cas9 gene editing in mice. Nucleic Acids Res 45:4606-4618
Jang, Younghoon; Wang, Chaochen; Zhuang, Lenan et al. (2017) H3K4 Methyltransferase Activity Is Required for MLL4 Protein Stability. J Mol Biol 429:2046-2054
He, Yunlong; Zhu, Wentao; Shin, Min Hwa et al. (2017) cFOS-SOX9 Axis Reprograms Bone Marrow-Derived Mesenchymal Stem Cells into Chondroblastic Osteosarcoma. Stem Cell Reports 8:1630-1644
Shin, Ha Youn; Wang, Chaochen; Lee, Hye Kyung et al. (2017) CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome. Nat Commun 8:15464
Pan, Haihui; Yan, Ye; Liu, Chengyu et al. (2017) The role of ZKSCAN3 in the transcriptional regulation of autophagy. Autophagy 13:1235-1238
Sweeney, Colin L; Choi, Uimook; Liu, Chengyu et al. (2017) CRISPR-Mediated Knockout of Cybb in NSG Mice Establishes a Model of Chronic Granulomatous Disease for Human Stem-Cell Gene Therapy Transplants. Hum Gene Ther 28:565-575
Xu, Zhe; Xing, Shaojun; Shan, Qiang et al. (2017) Cutting Edge: ?-Catenin-Interacting Tcf1 Isoforms Are Essential for Thymocyte Survival but Dispensable for Thymic Maturation Transitions. J Immunol 198:3404-3409
Shin, Min Hwa; He, Yunlong; Marrogi, Eryney et al. (2016) A RUNX2-Mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells. PLoS Genet 12:e1005884
Liu, Julia C; Liu, Jie; Holmström, Kira M et al. (2016) MICU1 Serves as a Molecular Gatekeeper to Prevent In Vivo Mitochondrial Calcium Overload. Cell Rep 16:1561-1573

Showing the most recent 10 out of 55 publications