In the past year, our core has assisted NHLBI investigators in successfully generating more than one dozen transgenic and knockout mouse lines. Four of these projects were completed using the recently developed targeted transgenic (also called site-specific transgenic) method. Our unit has also devoted a significant portion of our effort on developing new capabilities in the rapidly advancing genome engineering technologies. We have successfully used the ZFN (zinc finger nuclease), TALEN, and CRISPR/Cas9, method to edit the genome of induced pluripotent stem cell (iPSC) lines. We have also succeeded in producing knockout mouse lines using the ZFN method, and are currently using the TALEN and CRISPR/Cas9 methods to generate knockout, conditional knockout, and knockin mouse lines. We have also been involved in a range of stem cell related projects, including animal iPSC/ESC derivation, teratoma formation assay, mouse models of stem cell transplantation, and tissue engineering. Throughout the year, we have provided numerous consultations and technical assistances to scientists with in and out of NHLBI to assist them in conducting mouse molecular genetic research, including designing DNA constructs, searching database, collecting various stages of embryos, deriving mouse embryonic fibroblasts (MEF), collecting mouse organs, and reconstituting mouse lines using cryopreserved sperm. I am also a member of the NHLBI Animal Care and Use Committee (ACUC) for reviewing animal protocols and conducting animal facility inspections.

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National Heart, Lung, and Blood Institute
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Liu, Zhonghua; Vong, Queenie P; Liu, Chengyu et al. (2014) Borg5 is required for angiogenesis by regulating persistent directional migration of the cardiac microvascular endothelial cells. Mol Biol Cell 25:841-51
Luo, Yongquan; Liu, Chengyu; Cerbini, Trevor et al. (2014) Stable enhanced green fluorescent protein expression after differentiation and transplantation of reporter human induced pluripotent stem cells generated by AAVS1 transcription activator-like effector nucleases. Stem Cells Transl Med 3:821-35
Lin, Yongshun; Li, Xiao-Yan; Willis, Amanda L et al. (2014) Snail1-dependent control of embryonic stem cell pluripotency and lineage commitment. Nat Commun 5:3070
Liu, Chengyu; Xie, Wen; Gui, Changyun et al. (2013) Pronuclear microinjection and oviduct transfer procedures for transgenic mouse production. Methods Mol Biol 1027:217-32
Liu, Chengyu (2013) Strategies for designing transgenic DNA constructs. Methods Mol Biol 1027:183-201
Liu, Chengyu; Du, Yubin; Xie, Wen et al. (2013) Purification of plasmid and BAC transgenic DNA constructs. Methods Mol Biol 1027:203-15
Kaneko, Kotaro J; Kohn, Matthew J; Liu, Chengyu et al. (2010) The acrosomal protein Dickkopf-like 1 (DKKL1) is not essential for fertility. Fertil Steril 93:1526-32
Schieke, Stefan M; Ma, Mingchao; Cao, Liu et al. (2008) Mitochondrial metabolism modulates differentiation and teratoma formation capacity in mouse embryonic stem cells. J Biol Chem 283:28506-12
Pobezinskaya, Yelena L; Kim, You-Sun; Choksi, Swati et al. (2008) The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors. Nat Immunol 9:1047-54
Xue, Hai-Hui; Jing, Xuefang; Bollenbacher-Reilley, Julie et al. (2008) Targeting the GA binding protein beta1L isoform does not perturb lymphocyte development and function. Mol Cell Biol 28:4300-9

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