The NIH-HSCF has routinely grown hES cells on the NIH registry from August 2003. In the following year, most of the hES cells on the NIH registry were obtained, expanded in culture and analyzed by karyotype and FACS analysis. In the period to 8/2004, the central achievement of NIH-HSCF was to show that sub-clones of a hES cell line had a stable genome using a high-resolution genome scanning method. This evidence that hES cells can be grown for long periods without genetic change suggested that they might provide an important resource in the urgent quest to better understand and treat human disease. The next major focus was to grow the cell lines following a standard procedure. When they were first established, laboratories around the world used different procedures to obtain relatively few stable cell lines. This stage of the work was carried out in an international collaboration organized through the International Stem Cell Consortium. The ISCC is sponsored by the International Stem Cell Forum, a grouping of research agencies in several countries including Australia, Canada, Israel, Sweden, the United Kingdom and the United States. The ISCC defined a set of antibodies and a gene array as standard tools to assess hES cells. The ISCC also promoted standards to determine the safety of hES cell lines. At this stage, the NIH-HSCF was one of the few sites contributing multiple hES cells from different providers grown under standard conditions. The outcome of these studies was increased confidence that hES cells could be maintained in a similar state in laboratories around the world. It is important to point out that human ES cells are difficult to grow because they are poised to differentiate into all the cell types of the body. Based on other work in LMB/NINDS that defined the cellular and molecular basis for the self-renewal/differentiation switch, the NIH-SCF optimized growth conditions for undifferentiated hES cells. This information and gene expression data on different hES cell lines is accessible on the NIH Stem Cell Facility web site. Current work in the NIH-SCF is aimed at providing assurance that this efficient growth method applies to all human ES cells and induced pluripotent stem (iPS) cells. The strong similarity between human ES cells and iPS cells defined by high-resolution tools suggests the analysis of human pluripotent cells will be rapidly implemented in human genetics laboratories. In this effort, the data bases, cell banks and procedures developed in the NIH-SCF are a core resource for researchers at NIH and the wider community of researchers defining the genetic basis of human disease.

Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
2010
Total Cost
$815,975
Indirect Cost
City
State
Country
Zip Code
Jiang, Xueying; Detera-Wadleigh, Sevilla D; Akula, Nirmala et al. (2018) Sodium valproate rescues expression of TRANK1 in iPSC-derived neural cells that carry a genetic variant associated with serious mental illness. Mol Psychiatry :
Chen, Kevin G; Mallon, Barbara S; Park, Kyeyoon et al. (2018) Pluripotent Stem Cell Platforms for Drug Discovery. Trends Mol Med 24:805-820
Ou, Jingxing; Ball, John M; Luan, Yizhao et al. (2018) iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications. Cell 173:851-863.e16
Vallabhaneni, Haritha; Lynch, Patrick J; Chen, Guibin et al. (2018) High Basal Levels of ?H2AX in Human Induced Pluripotent Stem Cells Are Linked to Replication-Associated DNA Damage and Repair. Stem Cells :
Nandal, Anjali; Mallon, Barbara; Telugu, Bhanu P (2017) Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System. J Vis Exp :
Horikawa, Izumi; Park, Kye-Yoon; Isogaya, Kazunobu et al. (2017) ?133p53 represses p53-inducible senescence genes and enhances the generation of human induced pluripotent stem cells. Cell Death Differ 24:1017-1028
Lin, Yongshun; Linask, Kaari L; Mallon, Barbara et al. (2016) Heparin Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes. Stem Cells Transl Med :
Bhadriraju, Kiran; Halter, Michael; Amelot, Julien et al. (2016) Large-scale time-lapse microscopy of Oct4 expression in human embryonic stem cell colonies. Stem Cell Res 17:122-9
Lee, Chun-Ting; Chen, Jia; Kindberg, Abigail A et al. (2016) CYP3A5 Mediates Effects of Cocaine on Human Neocorticogenesis: Studies using an In Vitro 3D Self-Organized hPSC Model with a Single Cortex-Like Unit. Neuropsychopharmacology :
Lee, Chun-Ting; Bendriem, Raphael M; Kindberg, Abigail A et al. (2015) Functional consequences of 17q21.31/WNT3-WNT9B amplification in hPSCs with respect to neural differentiation. Cell Rep 10:616-32

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