The CMB Infectious Disease Pathogenesis Section assisted Principal Investigators with experimental design, tissue collection and preparation, interpretation of histopathology findings, and assistance with manuscript preparation. CMB Research Support Specialists also provided technical expertise and support to NIAID Principal Investigators, assisting them with true cage to beachside support. Both pathology and technical proficiency resulted in numerous co-authorship opportunities. The CMB has completed the challenge of bringing a gnotobiotic facility on line. The gnotobiotic facility consists of 12 bio-exclusion units designed to keep the mice from becoming colonized with any adventitious microorganisms. Germ-free mice are free of all aerobic and anaerobic organisms with the possible exception of endogenous viruses. Breeding colonies and mice on study are maintained in semi-rigid isolators provided with HEPA-filtered air and autoclaved food, bedding, and supplies. Strict SOPs are followed to maintain the mice in a germ-free state. The CMB has an active Assisted Reproduction Technologies and Cryopreservation unit. Last year the ARTiC group had 68 sperm cryo request for 23 PIs and 2 requests for 1 PI inconjunction with Taconic. This year, the section had 30 requests from 14 PIs with 5 reuqeusts still in the pipeline. Last year, there were 40 Embryo Cryo requests from 14 PIs that resulted in 6607 empryos. There was 1 Taconic project from 1 PI that resulted in 312 embryos. This year, there were 8 requests from 8 PIs that resulted in 946 embryos and 2 Taconic projects that resulted in 240 embryos. There are 4 projects still in the pipeline for FY13. Last year there were 30 rederivation requests, this year there were 18. ARTiC ahd 3 requests for Transgenic mice in 2012 and 1 request in 2013. A mission of the Comparative Medicine Branch (CMB) is to provide support to the emerging and re-emerging infectious disease research conducted within the NIAID/DIR Animal Biosafety Level (ABSL) 3 facilities. Members of the Comparative Medicine Branch (CMB) along with a Division of Occupational Health and Safety (DOHS) representative have submitted a manuscript to the Journal of the American Association for Laboratory Animal Science (JAALAS) providing a background of laws, regulations, and an overview of programmatic requirements pertaining to the use of Select Agents. The manuscript was published in JAALAS in 2012.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Zip Code
Vaughan, Kelli L; Szarowicz, Mark D; Herbert, Richard L et al. (2014) Comparison of anesthesia protocols for intravenous glucose tolerance testing in rhesus monkeys. J Med Primatol 43:162-8
Maximova, Olga A; Speicher, James M; Skinner, Jeff R et al. (2014) Assurance of neuroattenuation of a live vaccine against West Nile virus: a comprehensive study of neuropathogenesis after infection with chimeric WN/DEN4?30 vaccine in comparison to two parental viruses and a surrogate flavivirus reference vaccine. Vaccine 32:3187-97
Mattison, Julie A; Wang, Mingyi; Bernier, Michel et al. (2014) Resveratrol prevents high fat/sucrose diet-induced central arterial wall inflammation and stiffening in nonhuman primates. Cell Metab 20:183-90
Knowlen, Grant G; Weller, Richard E; Perry, Ruby L et al. (2013) Hypertrophic cardiomyopathy in owl monkeys (Aotus spp.). Comp Med 63:279-87
Michaud, C R; Ragland, D R; St Claire, M C et al. (2013) Mucinous cystadenoma in the lung of a captive-born moustached tamarin (Saguinus mystax). J Comp Pathol 149:376-80
Via, Laura E; Weiner, Danielle M; Schimel, Daniel et al. (2013) Differential virulence and disease progression following Mycobacterium tuberculosis complex infection of the common marmoset (Callithrix jacchus). Infect Immun 81:2909-19
Waisberg, Michael; Lin, Christina K; Huang, Chiung-Yu et al. (2013) The impact of genetic susceptibility to systemic lupus erythematosus on placental malaria in mice. PLoS One 8:e62820
Gozalo, A S; Zerfas, P M; Starost, M F et al. (2013) Pancreatic endocrine tumour with disseminated pulmonary thromboembolism in an owl monkey (Aotus nancymae). J Comp Pathol 149:132-6
Boonnak, Kobporn; Vogel, Leatrice; Orandle, Marlene et al. (2013) Antigen-activated dendritic cells ameliorate influenza A infections. J Clin Invest 123:2850-61
Fornek, Jamie L; Gillim-Ross, Laura; Santos, Celia et al. (2009) A single-amino-acid substitution in a polymerase protein of an H5N1 influenza virus is associated with systemic infection and impaired T-cell activation in mice. J Virol 83:11102-15

Showing the most recent 10 out of 37 publications