The interplay between deformable microstructure and macroscale flow dynamics is a long-standing problem in particulate and multiphase fluid dynamics. The major challenge stems from the free-boundary nature of the particle interface. Lipid bilayer membranes that envelop cells are particularly complex interfaces because of their unique mechanics: the molecularly thin membrane is a highly-flexible incompressible uid sheet. As a result, particles made of closed lipid bilayers (cells and vesicles) exhibit richer dynamics than would capsules and drops. The objective of the proposed research is to understand the fluid-bilayer membrane coupling, with the long-term goal of explaining the non-equilibrium dynamics of suspensions of soft particles, such as biological cells. To achieve these goals, the PI will integrate ideas from fluid dynamics and biophysics to build a unified theoretical framework that will elucidate the interrelation between membrane deformation and composition, fluid motion, and external fields. This transformative approach encompasses three fundamental non-equilibrium problems: flows, electric fields, and multicomponent membranes. The proposed combination of theoretical, numerical, and experimental work will quantify vesicle deformation, orientation, and motion in external fields.

Intellectual Merit:

This CAREER plan sets forth the first systematic study of non-equilibrium bilayer membranes. The proposed research will advance our understanding of the interplay of physical processes at the nano-scale (e.g., membrane thermal undulations, poration), micro-scale (e.g., single vesicle deformation), and macro-scale (e.g., rheology of vesicle suspensions). Moreover, it will shed light on several controversial topics, e.g., the diversity of vesicle behaviors in linear and complex flows, the unusual shapes of vesicles in electric fields, and the viscosity of vesicle suspensions.

Broader Impacts:

The outcomes of the research will dramatically advance the field of biomicrouidics in multiple directions. First, the new knowledge will establish a sound basis for innovative designs of micro- and nano-devices built from lipid bilayers, such as networks of nanotubes and vesicle containers. Second, the proposed studies will uncover new general features of cell-force interactions, which will likely impact a wide range of applications, including cell electro-manipulation and targeted drug and gene delivery. The project has a strong interdisciplinary nature and combines fundamental knowledge across many fields, including biology, physics, and engineering. The international and interdisciplinary collaborations initiated by the PI will facilitate communication among scientists and engineers in different research and geographic areas. As a female faculty member, the PI will serve as a mentor and a role model to women as part of the Women in Science Project at Dartmouth, and will reach out to other under-represented minorities in science and engineering. She will collaborate with the Montshire Science Museum and Dartmouth's Outreach Office to develop educational programs for under-resourced schools in poor and rural areas. Education. The PI will develop new courses, e.g., Cellular and Molecular Biomechanics, which fuse engineering and biophysics. Such classes will enhance the curriculum at Dartmouth College, where engineering research and education in complex biological systems is in its early stages. The PI's teaching will emphasize the importance of analytical skills and theoretical framework in solving the real-world problems that students will encounter in their professional lives. She is co-organizing a summer school on Complex and Bio-Fluids Flows," for which the lectures will be web-published, to promote the transfer of knowledge between disciplines and generations. The PI will educate the public about the beauty of science by showcasing her Lab at the annual Thayer School of Engineering open house, which attracts children, their families, and local engineering enthusiasts.

Project Report

This interdisciplinary project aimed at understanding and mimicking the design of the biological cell. The PI’s research integrated engineering and biophysical approaches in order to answer questions such as the link between the unusual mechanics of the red blood cell membrane and blood viscosity. The research advances fundamental knowledge in cell biomechanics, and it is helpful to biomedical applications such as targeted drug delivery. One of the central issues investigated by the PI involved the mechanisms of how the cell membrane is deformed by fluid or electrical stresses. This problem was studied through theoretical modeling, numerical simulations, and experiments. The research outcomes were published in 19 papers and a book chapter. This award also supported the PI’s efforts in teaching and training the next generation of engineers, physicists, and biologists to effectively cooperate on problems that cut across these areas. The PI has actively recruited and served as a role model for engineering and physics students and researchers. One graduate and 8 undergraduate students (6 of them female) were engaged in the research supported by this ward. The PI has taught a broad spectrum of courses in fluid dynamics and biophysics, and she has co-organized two summers schools on Complex- and Bio-Fluid Dynamics.

Project Start
Project End
Budget Start
2010-10-01
Budget End
2014-02-28
Support Year
Fiscal Year
2011
Total Cost
$349,274
Indirect Cost
Name
Brown University
Department
Type
DUNS #
City
Providence
State
RI
Country
United States
Zip Code
02912