In order to fully understand how the sperm reaches the egg, we need to have an understanding of how the sperm is able to modify its waveform in response to the surrounding environment. Since sperm motility is an emergent property of a complex system, we need to have multiscale models that account for chemical signaling, mechanics, and hydrodynamics. For marine invertebrate sperm, a chemoattractant is able to guide sperm to the egg by initiating a signaling pathway that results in an increase in calcium. This increase in calcium modifies the waveform, and ultimately the trajectory. This project will develop a hierarchy of models that will be used to investigate the relevant biochemistry of chemotaxis, how and where calcium is acting to modify the flagellar waveform, and how this couples to trajectories that allow the sperm to reach the egg. A model for the chemotactic signaling pathway will be developed and the Geometric Clutch Hypothesis will be implemented to have an accurate model of how calcium effects dynein arm activation (active force and torque generators in the axoneme of the flagellum). Multiscale models will be developed to couple the relevant biochemistry at the cellular level to trajectories and flagellar bending at the macroscale. In order to accurately account for nonplanar bending of the flagellum in a 3-dimensional fluid governed by the Stokes equations, we will develop a new regularized formulation of the generalized immersed boundary method. This method will provide a framework to numerically solve a coupled system that includes an immersed structure in a viscous fluid, where the force and torque that the sperm flagellum exerts on the fluid can depend on the biochemistry.

Through the development of integrative, multiscale models, we will examine how chemotaxis and the resulting increase in calcium concentration within the flagellum enables invertebrate sperm to reach and fertilize the egg in a marine environment. The modeling framework that will be developed will allow us to test different hypotheses about where calcium is acting in the flagellum to understand how this couples to emergent waveforms and trajectories of sperm. This research lies at the interface of mathematics and biology, as it is necessary to account for the relevant biochemistry, mechanics, and hydrodynamics to model sperm motility. Students involved in this research project will receive interdisciplinary training and will gain experience in model development based on the relevant biology and computational aspects of the project. Although the main focus of this project is on studying aspects of invertebrate sperm motility, the new numerical method that will be developed will also be applicable to study aspects of motility in other microorganisms such as Escherichia coli. The aim of this project is to develop mathematical models to understand how sea urchin sperm reach and fertilize the egg in a marine environment. This research will also shed light onto aspects of mammalian sperm motility and will be relevant to diagnosing infertility and developing contraceptives.

Agency
National Science Foundation (NSF)
Institute
Division of Mathematical Sciences (DMS)
Type
Standard Grant (Standard)
Application #
1122461
Program Officer
Mary Ann Horn
Project Start
Project End
Budget Start
2011-09-01
Budget End
2014-08-31
Support Year
Fiscal Year
2011
Total Cost
$99,707
Indirect Cost
Name
Worcester Polytechnic Institute
Department
Type
DUNS #
City
Worcester
State
MA
Country
United States
Zip Code
01609