Changes in brain development can lead to changes in adult behavior. Such developmental alterations may be due to changes in the environment, a phenomenon known as phenotypic plasticity. Phenotypic plasticity is a ubiquitous phenomenon but many questions about its underlying molecular basis remain unanswered. To address these questions, gene expression in the brains of individuals exposed to different environmental conditions during development will be compared. The butterfly Bicyclus anynana, a seasonally plastic butterfly in which the temperature during development determines the adult courtship behavioral phenotype, will be used for these experiments. When reared under warm conditions (27?aC), that mimic the natural wet season experienced by wild B. anynana, adult males court females avidly. Male butterflies that are reared under cool conditions (17?aC), that mimic the natural dry season, are passive and court females significantly less. This study will use the sequencing technology known as RNA seq to measure gene expression in the brains of butterflies exposed to the different temperatures during development. This will enable identification of genes that may play a role in the temperature dependant development of the brain and behavior in B. anynana. Broader Impacts: This study is expected to shed new light on how the environment affects behavior. This is an important goal of both evolutionary biologists, who seek to understand how organisms produce adaptive behaviors in changing environments, and neuroscientists, who seek to understand the physiological basis of behavior. Thus, this project promotes collaboration between branches of biology with diverse perspectives, skills, and techniques. The results of this research will be made available to the general public through a website that will convey information in a jargon-free way that is accessible to high-school students, as well as through presentations at local schools that highlight the contribution of women in science.

Project Report

The environment can have a major impact on the development of organismal traits. Understanding how this is achieved is particularly important in light of the potential implications of anthropogenic climate change. In order to better understand how the environment can modify the traits of an organism, we studied the behavior of butterflies that exhibit different traits in adulthood after they develop at different temperatures. Specifically, we studied the African butterfly Bicyclus anynana (B. anynana), a butterfly that develops in nature during both a cool dry season and a warm wet season. Extensive research on this butterfly has shown that B. anynana that develop in the dry season exhibit different physical and behavioral characteristics than those that develop in the wet season. In order to gain a deeper understanding of how temperature experienced during development influences adult behavior, we first identified the specific time period during development when temperature had an impact on behavior. We found that low temperatures experienced during the pupal stage of development reduced adult courtship behavior, regardless of temperature experienced by the adult butterfly. This suggests that temperature has a latent effect on behavior in B. anynana. With this information, we then sought to understand how temperature during the pupal stage modified the physiology of the butterfly. We found that differences in steroid hormone levels, but not steroid receptor expression in the brain, were present at the temperature sensitive stage of development. In particular, butterflies reared at low temperatures had lower levels of these hormones. We confirmed a causal role for the steroid hormone levels by performing hormone treatment experiments that decoupled the hormone level from the rearing temperature. Finally, in order to understand how the steroid hormone levels influenced the developing brains of the butterflies, we conducted an RNA-Seq experiment. We compared gene expression levels in the brains of butterflies reared at the dry and wet season temperatures, with those reared at dry season temperatures who received a hormone treatment that artificially mimicked the hormonal physiology of butterflies reared at warm temperatures. Using this approach, we were able to identify several candidate genes that could play a role in regulating the development of different behaviors in butterflies that experienced different environmental or physiological conditions. We are currently in the process of further investigating how these candidate genes may influence the development of the brain in a way that leads B. anynana butterflies to exhibit different levels of courtship behavior after exposure to different environmental conditions. In order to broadly disseminate information about how the environment influences the development of organismal traits (including, but not exclusively, butterflies) A. Bear visited schools, museums, and camps and engaged young people in activities and demonstrations that showed how the environment can influence the development and the evolution of traits in organisms. A. Bear did this in collaboration with the Evolution Outreach Group (http://evolutionoutreach.org).

Agency
National Science Foundation (NSF)
Institute
Division of Integrative Organismal Systems (IOS)
Type
Standard Grant (Standard)
Application #
1110523
Program Officer
Michelle M. Elekonich
Project Start
Project End
Budget Start
2011-06-15
Budget End
2014-05-31
Support Year
Fiscal Year
2011
Total Cost
$14,310
Indirect Cost
Name
Yale University
Department
Type
DUNS #
City
New Haven
State
CT
Country
United States
Zip Code
06520