Preliminary studies show that in L1M1863 (human colon cancer) cells, miR-21 and miR-31 are upregulated during TGF-P-induced epithelial-mesenchymal transition (EMT). This proposal aims to explore the significance of miR-21 and miR-31 as effectors or modifiers of TGF-3 signaling in the context of EMT by 1) modulating the function of these miRNAs and evaluating the effect on EMT marker expression and 2) identifying mRNA targets of miR-21 and miR-31 that are relevant for EMT. The experiments.proposed here are a new angle to study cellular regulation by TGF-P and will significantly advance current understanding of how TGF-(3 regulates growth and differentiation of many types of cells. Abnormalities in TGF-(3 signaling are strongly implicated in the development of many diseases, particularly cancer. A better knowledge of cellular regulation by TGF-p is essential to understand mechanisms of cancer development, and provides vital information for the design of therapeutic interventions.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31CA142216-02
Application #
8101248
Study Section
Special Emphasis Panel (ZRG1-CB-N (29))
Program Officer
Bini, Alessandra M
Project Start
2009-07-01
Project End
2011-06-30
Budget Start
2010-07-01
Budget End
2011-06-30
Support Year
2
Fiscal Year
2010
Total Cost
$41,380
Indirect Cost
Name
University of Massachusetts Medical School Worcester
Department
Other Basic Sciences
Type
Schools of Medicine
DUNS #
603847393
City
Worcester
State
MA
Country
United States
Zip Code
01655
Cottonham, Charisa L; Kaneko, Satoshi; Xu, Lan (2010) miR-21 and miR-31 converge on TIAM1 to regulate migration and invasion of colon carcinoma cells. J Biol Chem 285:35293-302