This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A novel F. hepatica Ag was recently cloned and characterized. It is a member of the F. hepatica saposin-like protein family which is expressed by parasite at early stages of infection and when it is injected in rabbits induce a strong resistance to a challenge infection. Previous immunization studies in mice have revealed that FhSAP-2 induce a mixed Th1/Th2 response, which is slightly polarized to Th1 when is delivered as DNA vaccine. It has been demonstrated that in chronic fascioliasis the infection is associated to Th2 response. However, in animals than develop a natural resistance to infection high levels of IgG2 and IFNg characteristic of a Th1 type response have been observed. Thus, the Th1 response appears to be necessary for inducing resistance to infection. A previous result obtained from our group suggests that FhSAP-2 is a Fasciola/Schistosoma cross-reactive antigen. Because of the heterologous immunity between both species has been demonstrated it is possible that FhSAP-2 could form part of a multi-component vaccine against both diseases. Thus, FhSAP-2 has become in a novel promising vaccine candidate that could also constitute the base for a dual-Fasciola/Schistosoma vaccine. Additional preliminary results need to be compiled before to initiate large vaccination trials. We previously demonstrated that FhSAP-2 induces significant protection against a challenged infection. However, in that study we failed to determine whether this protection is Th1-biased or Th2-biased. In the mouse study we evaluated the immune response after immunization with FhSAP-2 and showed that Th1-biased response may be predominant, but failed to determine whether this immunization protocol conferred any protection. We also demonstrated that FhSAP-2 is a Fasciola/Schistosoma cross-reactive Ag. However, the simple cross-reactivity should not be the only criterion for developing a vaccine against schistosomiasis. The current proposal intends to accomplish two specific aims. In the Aim1 we will characterize the immune responses to FhSAP-2 produced in mice and we will determine if this protection is Th1-biased or Th2-biased. In the Aim-2 we will determine whether sera from mouse immunized with irradiated cercariae (the gold standard vaccine for schistosomiasis) recognize FhSAP-2 and whether a homolog FhSAP-2 exits in S. mansoni. The results to be obtained in the present pilot project will serve to submit an RO1 project in short period of time.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Centers in Minority Institutions Award (G12)
Project #
2G12RR003051-26
Application #
8357165
Study Section
Special Emphasis Panel (ZRR1-RI-B (02))
Project Start
2011-09-15
Project End
2012-07-31
Budget Start
2011-09-15
Budget End
2012-07-31
Support Year
26
Fiscal Year
2011
Total Cost
$92,126
Indirect Cost
Name
University of Puerto Rico Med Sciences
Department
Pharmacology
Type
Schools of Medicine
DUNS #
948108063
City
San Juan
State
PR
Country
United States
Zip Code
00936
Rullán-Lind, Carlos; Pietri, Ruth B; Vázquez-Cintrón, Melvin et al. (2018) Fused dimerization increases expression, solubility, and activity of bacterial dehydratase enzymes. Protein Sci 27:969-975
Martínez-Rivera, Freddyson J; Barreto-Estrada, Jennifer L (2018) Reply to: Does High-Frequency Deep Brain Stimulation in Dorsal Regions of the Ventral Striatum Impair Extinction of Morphine-Induced Place Preference? Biol Psychiatry 83:e21
Ebersole, J L; Dawson 3rd, D A; Emecen Huja, P et al. (2018) Age and Periodontal Health - Immunological View. Curr Oral Health Rep 5:229-241
Ebersole, Jeffrey L; Orraca, Luis; Kensler, Terry B et al. (2018) Periodontal disease susceptible matrilines in the Cayo Santiago Macaca mulatta macaques. J Periodontal Res :
Ramírez, Maite; Santos, Saritza; Martínez, Osmarie et al. (2018) Characterization of the immune response elicited by the vaccinia virus L3 protein delivered as naked DNA. Vaccine 36:2049-2055
Vaquer-Alicea, Ana Del C; Vázquez-Torres, Rafael; Devarie-Hornedo, Marcos et al. (2018) aPKC-Mediated Persistent Increase in AMPA/NMDA Ratio in the VTA Participates in the Neuroadaptive Signal Necessary to Induce NAc Synaptic Plasticity After Cocaine Administration. Neuroscience 392:129-140
Roche-Lima, Abiel; Carrasquillo-Carrión, Kelvin; Gómez-Moreno, Ramón et al. (2018) The Presence of Genotoxic and/or Pro-inflammatory Bacterial Genes in Gut Metagenomic Databases and Their Possible Link With Inflammatory Bowel Diseases. Front Genet 9:116
Colón-Cruz, Luis; Kristofco, Lauren; Crooke-Rosado, Jonathan et al. (2018) Alterations of larval photo-dependent swimming responses (PDR): New endpoints for rapid and diagnostic screening of aquatic contamination. Ecotoxicol Environ Saf 147:670-680
Jang, Sehwan; Lewis, Taber S; Powers, Corey et al. (2017) Elucidating Mitochondrial Electron Transport Chain Supercomplexes in the Heart During Ischemia-Reperfusion. Antioxid Redox Signal 27:57-69
Díaz-Ayala, Ramonita; Torres-González, Lisa; Pietri, Ruth et al. (2017) Engineered (Lys)6-Tagged Recombinant Sulfide-Reactive Hemoglobin I for Covalent Immobilization at Multiwalled Carbon Nanotubes. ACS Omega 2:9021-9032

Showing the most recent 10 out of 414 publications