Continue work in the testing of radiosensitivity and DNA repair in skin fibroblasts obtained from patients known to be at high risk of cancer. The Contractor will provide technical and professional services to perform in vitro studies. In a supportive role, the Contractor will carry out assays previously developed and refined. The Contractor in the research capacity will engage in the application and analysis of research assays. It is expected that approximately 30 strains will be tested annually for their response to 60Co gamma-ray radiation and to ultraviolet (UV) radiation (254 mm).

National Institute of Health (NIH)
Division of Cancer Epidemiology And Genetics (NCI)
Research and Development Contracts (N01)
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Chalk River Nuclear Laboratories
Chalk River
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Mirzayans, R; Aubin, R A; Bosnich, W et al. (1995) Abnormal pattern of post-gamma-ray DNA replication in radioresistant fibroblast strains from affected members of a cancer-prone family with Li-Fraumeni syndrome. Br J Cancer 71:1221-30
Mirzayans, R; Sabour, M; Rauth, A M et al. (1993) Hyperresistance to 4-nitroquinoline 1-oxide cytotoxicity and reduced DNA damage formation in dermal fibroblast strains derived from five members of a cancer-prone family. Br J Cancer 68:838-44
Mirzayans, R; Middlestadt, M V; Paterson, M C (1992) Cytotoxic and mutagenic effects of methylnitrosourea in two human fetal fibroblast strains differing in O6-methylguanine-DNA methyltransferase activity. Carcinogenesis 13:1185-90
Marshall, R S; Paterson, M C; Rauth, A M (1991) DT-diaphorase activity and mitomycin C sensitivity in non-transformed cell strains derived from members of a cancer-prone family. Carcinogenesis 12:1175-80
Marshall, R S; Paterson, M C; Rauth, A M (1991) Studies on the mechanism of resistance to mitomycin C and porfiromycin in a human cell strain derived from a cancer-prone individual. Biochem Pharmacol 41:1351-60
Kuhnlein, U; Paterson, M C (1990) Increased uracil-DNA glycosylase, AP-DNA binding protein and deoxyribonuclease activities in tumor and SV40-transformed cell lines of human origin. Carcinogenesis 11:117-21
Liuzzi, M; Weinfeld, M; Paterson, M C (1989) Enzymatic analysis of isomeric trithymidylates containing ultraviolet light-induced cyclobutane pyrimidine dimers. I. Nuclease P1-mediated hydrolysis of the intradimer phosphodiester linkage. J Biol Chem 264:6355-63
Mirzayans, R; Smith, B P; Paterson, M C (1989) Hypersensitivity to cell killing and faulty repair of 1-beta-D-arabinofuranosylcytosine-detectable sites in human (ataxia-telangiectasia) fibroblasts treated with 4-nitroquinoline 1-oxide. Cancer Res 49:5523-9
Mirzayans, R; Sabour, M; Paterson, M C (1989) Bioreduction of 4-nitroquinoline 1-oxide in dysplastic nevus syndrome fibroblasts. Mutat Res 225:165-9
Marshall, R S; Paterson, M C; Rauth, A M (1989) Deficient activation by a human cell strain leads to mitomycin resistance under aerobic but not hypoxic conditions. Br J Cancer 59:341-6

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