Abstract

The core consists of a central cell culture and hybridoma facility. This unit will also maintain facilities for storage of cell lines and hybridomas at -80 C or in liquid nitrogen. Service contracts for items of equipment which are regularly utilized by project investigators will be carried in the core budget. Other costs such as those for glassware washing and sterilization, the purchase of some cryopreservation and centrifugation equipment, as well as some animal purchase and maintenance costs will be borne by the core. This unit will employ two technicians, one full time and the other 20% time. Janet Gilmore, who will be employed full time, is described as an experienced research technician. She will be responsible for the cell culture component of the hybridoma projects described by Drs. Oldstone, Wilson and Fujinami. She will also carry a number of cell lines necessary for the conduct of Dr. Southern's and Dr. Oldstone's project. Charles Gillette, the 20% time research technician, will order supplies and prepare/order medium for the group.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG004342-17
Application #
6200954
Study Section
Project Start
1999-12-01
Project End
2000-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
17
Fiscal Year
2000
Total Cost
$149,274
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Oldstone, Michael B A (2014) Molecular mimicry: its evolution from concept to mechanism as a cause of autoimmune diseases. Monoclon Antib Immunodiagn Immunother 33:158-65
Priola, Suzette A; Ward, Anne E; McCall, Sherman A et al. (2013) Lack of prion infectivity in fixed heart tissue from patients with Creutzfeldt-Jakob disease or amyloid heart disease. J Virol 87:9501-10
Siggs, Owen M; Cruite, Justin T; Du, Xin et al. (2012) Disruption of copper homeostasis due to a mutation of Atp7a delays the onset of prion disease. Proc Natl Acad Sci U S A 109:13733-8
Sun, Binggui; Halabisky, Brian; Zhou, Yungui et al. (2009) Imbalance between GABAergic and Glutamatergic Transmission Impairs Adult Neurogenesis in an Animal Model of Alzheimer's Disease. Cell Stem Cell 5:624-33
Trifilo, Matthew J; Sanchez-Alavez, Manuel; Solforosi, Laura et al. (2008) Scrapie-induced defects in learning and memory of transgenic mice expressing anchorless prion protein are associated with alterations in the gamma aminobutyric acid-ergic pathway. J Virol 82:9890-9
Biasini, Emiliano; Seegulam, M Esa; Patti, Brianna N et al. (2008) Non-infectious aggregates of the prion protein react with several PrPSc-directed antibodies. J Neurochem 105:2190-204
Balch, William E; Morimoto, Richard I; Dillin, Andrew et al. (2008) Adapting proteostasis for disease intervention. Science 319:916-9
Trifilo, Matthew J; Ying, Ge; Teng, Chao et al. (2007) Chronic wasting disease of deer and elk in transgenic mice: oral transmission and pathobiology. Virology 365:136-43
Trifilo, Matthew J; Yajima, Toshitaka; Gu, Yusu et al. (2006) Prion-induced amyloid heart disease with high blood infectivity in transgenic mice. Science 313:94-7
Leclerc, E; Serban, H; Prusiner, S B et al. (2006) Copper induces conformational changes in the N-terminal part of cell-surface PrPC. Arch Virol 151:2103-9

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