The central aim of this proposal is to understand the biological effects of Alzheimer amyloid precursor protein (APP) expression in transgenic (Tg) mice, which we believe will in turn reflect important aspects of the biology of Alzheimer's disease (AD), whose fundamental etiology is unknown. To accomplish this we propose to build upon our knowledge of Tg mice, designated Tg(HuAPP695.K670N/M671L)2576 (or Tg2576), expressing the 695 amino-acid isoform of APP with mutations at amino acids 670 and 671 (APP-77O numbering) found in a large Swedish family with Alzheimer's disease. These mice have age-related deficits in learning and memory associated with the appearance of classic senile plaques. They shall enable us to extend our understanding of the pathophysiology and neurobiology of AD using genetic (Drs. George Carlson and Karen Hsiao, Projects 2 and 1), anatomical and molecular pathological (Dr. Brad Hyman, Project 3), and physiological and behavioral methods (Dr. Paul Chapman, Project 4). By comparing these mice with transgenic mice expressing equivalent levels of wild-type APP-695 (Dr. Steven Younkin, Core B) we shall be able to ascertain which behavioral, neurophysiological and neuropathological abnormalities are related to an overall increase in brain APP expression and which are due to APP cleavage products, including Abeta (Dr. Steven Younkin, Core B). Moreover, we shall attempt to determine the form (soluble or insoluble) and location (intracellular or extracellular) of A beta that best corresponds to specific phenotypic traits. Modifications of the transgene used in Tg2576 mice may also allow us to understand the role of aging in triggering the deficits we observe and delineate temporal windows during which deficits can be reversed (Dr. Karen Hsiao, Project 1). If the basic biological mechanisms for the age-related cognitive deficits in AD are discovered, more effective drugs than are currently available may be designed to circumvent them.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG015453-02
Application #
6169164
Study Section
National Institute on Aging Initial Review Group (NIA)
Program Officer
Snyder, D Stephen
Project Start
1999-06-15
Project End
2004-04-30
Budget Start
2000-07-01
Budget End
2001-04-30
Support Year
2
Fiscal Year
2000
Total Cost
$1,070,201
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Neurology
Type
Schools of Medicine
DUNS #
168559177
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
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