Core C - James E. HaydenThe Microscopy and Imaging Core will be responsible for expert microscopy, imaging and analytical servicesto support the specific needs of the individual program projects and will consist primarily of new, dedicatedequipment and personnel to support these aims. Services will include 1) Aging assays using repetitive yeastmother/daughter cell dissection and yeast tetrad dissection techniques, 2) High magnification microscopicimaging and data quantitation of immunofluorescence in cultured mammalian and yeast cells (fixed cells andreal-time imaging), 3) Brightfield microscopy and analysis of stained tissue sections, 4) High magnificationcounting and documentation of bud scars on yeast, 5) Liquid senescence assays using brightfield imagingand analysis, and 6) Access to additional imaging modalities and analysis software as needed byinvestigators. Core C represents an expansion of services offered in the Wistar Institute Microscopy Facility.This Facility does not currently have all of the instrumentation or personnel required to support the aims ofthe Program Project, so creation of an associated Microscopy and Imaging Core specifically for the needs ofthese projects is warranted. By incorporating into the Wistar Facility, the PO1 Core can take advantage ofexisting infrastructure, equipment and software rather than duplicate those items which would be usedinfrequently or are not cost effective to purchase exclusively for the PO1 Core, such as a confocalmicroscope. Core C will utilize the existing Wistar infrastructure for online scheduling and data managementas well as all administrative functions and supervision of the new Core. Microscopy services for the grant willbe provided by a dedicated, full time research assistant who will be trained and supervised by the WistarFacility to insure the highest quality results for program researchers.

National Institute of Health (NIH)
National Institute on Aging (NIA)
Research Program Projects (P01)
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Special Emphasis Panel (ZAG1-ZIJ-5 (J2))
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Wistar Institute
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