Core B. This Core will provide three separate but highly integrated functions, with each component representing a critical element to the P01's Projects. First is a biorepository function;a unit within the University of Florida's NIH funded CTSI Biorepository. Here, the Core will validate proper patient consenting, tissue procurement, sample processing, storage and distribution. Core B will ensure that high quality specimens are obtained through adherence to GLP procedures, computerized sample tracking, biobanking best practices, automated storage, as well as emergency response planning by trained staff. The Core will routinely store samples collected as part of the Program, including serum, plasma, PBMCs, DNA, RNA and urine (if ever deemed necessary). The samples will be securely managed using web-based software including the OnCore Biospecimen Management System that tracks patient consent, patient visits, collection and annotation of specimens, specimen processing, storage, quality assurance and distribution of samples. With this, the Core should provide Projects 1 and 2 with a uniformly processed aliquot of a sample in order to optimize multivariate analysis of data across the Program. A second activity for this Core involves service by the Immunology/Pathology Laboratory. Here, as a routine analysis for subjects enrolled in this study, the Core will provide autoantibody (i.e., GADA, IA-2A, and ZnT8A) analysis using commercially available kits, and mlAA by standard assay. The Core's performance in the Diabetes Autoantibody Standardization Program routinely scores with high sensitivity/specificity. Additional services will include HLA typing and overseeing the highly innovative immunochip analysis. Finally, the Core will provide Molecular Pathology services. This laboratory serves as a key element to the JDRF Network for Pancreatic Organ donors with Diabetes (nPOD) effort. Core B will provide paraffin and frozen section preparation, histology, quantitative morphology, immunolocalization (IHC, IF, ISH) and e-storage of microscopic images. We see Core B as both an innovative and highly valuable unit only available through a PPG Core mechanism.
Core B will provide quality samples, as well as a comprehensive laboratory data storage/analysis function;both deemed essential to high quality research. In addition, the Core has established standard operating procedures (GLP grade) and trained personnel to ensure high quality performance. Core activities will also help integrate Projects within the POI;increasing its value and potential to deliver on its goals.
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|Smith, Mia J; Rihanek, Marynette; Wasserfall, Clive et al. (2018) Loss of B-Cell Anergy in Type 1 Diabetes Is Associated With High-Risk HLA and Non-HLA Disease Susceptibility Alleles. Diabetes 67:697-703|
|Perry, Daniel J; Wasserfall, Clive H; Oram, Richard A et al. (2018) Application of a Genetic Risk Score to Racially Diverse Type 1 Diabetes Populations Demonstrates the Need for Diversity in Risk-Modeling. Sci Rep 8:4529|
|Chen, Yi-Guang; Mathews, Clayton E; Driver, John P (2018) The Role of NOD Mice in Type 1 Diabetes Research: Lessons from the Past and Recommendations for the Future. Front Endocrinol (Lausanne) 9:51|
|Kusmartseva, Irina; Beery, Maria; Philips, Tiffany et al. (2018) Hospital time prior to death and pancreas histopathology: implications for future studies. Diabetologia 61:954-958|
|Ratiu, Jeremy J; Racine, Jeremy J; Hasham, Muneer G et al. (2017) Genetic and Small Molecule Disruption of the AID/RAD51 Axis Similarly Protects Nonobese Diabetic Mice from Type 1 Diabetes through Expansion of Regulatory B Lymphocytes. J Immunol 198:4255-4267|
|Delitto, Daniel; Delitto, Andrea E; DiVita, Bayli B et al. (2017) Human Pancreatic Cancer Cells Induce a MyD88-Dependent Stromal Response to Promote a Tumor-Tolerant Immune Microenvironment. Cancer Res 77:672-683|
|Posgai, Amanda L; Wasserfall, Clive H; Kwon, Kwang-Chul et al. (2017) Plant-based vaccines for oral delivery of type 1 diabetes-related autoantigens: Evaluating oral tolerance mechanisms and disease prevention in NOD mice. Sci Rep 7:42372|
|Sebastiani, Guido; Ventriglia, Giuliana; Stabilini, Angela et al. (2017) Regulatory T-cells from pancreatic lymphnodes of patients with type-1 diabetes express increased levels of microRNA miR-125a-5p that limits CCR2 expression. Sci Rep 7:6897|
|O'Kell, Allison L; Wasserfall, Clive; Catchpole, Brian et al. (2017) Comparative Pathogenesis of Autoimmune Diabetes in Humans, NOD Mice, and Canines: Has a Valuable Animal Model of Type 1 Diabetes Been Overlooked? Diabetes 66:1443-1452|
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