Abstract

The objectives of this Core are to aid the efforts to map DNA damage and repair at high resolution by facilitating efficient use of existing LMPCR technology and helping to improve the technology. To do this we will (1) have a coordinator, aided by a data manager, provide service and coordination for use of extant LMPCR and other techniques by members of the Program; and (2) have a development-technician further develop and at least partially automate LMPCR.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA069449-03
Application #
6103192
Study Section
Project Start
1998-09-01
Project End
2000-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
City of Hope/Beckman Research Institute
Department
Type
DUNS #
City
Duarte
State
CA
Country
United States
Zip Code
91010

  Publications

Sanders, M H; Bates, S E; Wilbur, B S et al. (2004) Repair rates of R-band, G-band and C-band DNA in murine and human cultured cells. Cytogenet Genome Res 104:35-45
Dai, S M; O'Connor, T R; Holmquist, G P et al. (2002) Ligation-mediated PCR: robotic liquid handling for DNA damage and repair. Biotechniques 33:1090-7
Komura , J; Ikehata, H; Hosoi, Y et al. (2001) Mapping psoralen cross-links at the nucleotide level in mammalian cells: suppression of cross-linking at transcription factor- or nucleosome-binding sites. Biochemistry 40:4096-105
Cloutier, J F; Castonguay, A; O'Connor, T R et al. (2001) Alkylating agent and chromatin structure determine sequence context-dependent formation of alkylpurines. J Mol Biol 306:169-88
Chen, J Z; Smith, L; Pfeifer, G P et al. (2001) Fluorescence-based directed termination PCR: direct mutation characterization without sequencing. Nucleic Acids Res 29:E17
Miao, F; Bouziane, M; Dammann, R et al. (2000) 3-Methyladenine-DNA glycosylase (MPG protein) interacts with human RAD23 proteins. J Biol Chem 275:28433-8
Bouziane, M; Miao, F; Bates, S E et al. (2000) Promoter structure and cell cycle dependent expression of the human methylpurine-DNA glycosylase gene. Mutat Res 461:15-29
Pfeifer, G P (2000) p53 mutational spectra and the role of methylated CpG sequences. Mutat Res 450:155-66
Szabo, P E; Pfeifer, G P; Miao, F et al. (2000) Improved in vivo dimethyl sulfate footprinting using AlkA protein: DNA-protein interactions at the mouse H19 gene promoter in primary embryo fibroblasts. Anal Biochem 283:112-6
Chen, J Z; Qiu, J; Shen, B et al. (2000) Mutational spectrum analysis of RNase H(35) deficient Saccharomyces cerevisiae using fluorescence-based directed termination PCR. Nucleic Acids Res 28:3649-56

Showing the most recent 10 out of 33 publications