Abstract

All three research projects in this grant proposal share the common goal in unraveling the developmental and oncogenic functions of the Ets family of transcription factors. Although different systems are used in the individual projects, many experimental approaches are common to all. One underlying philosophy of this grant proposal is that the Center for Molecular and Structural Biology (CMSB) can provide share facility and technical expertise to the proposed research projects; and by consolidating the resources we will be able to afford efficient and economical support for the objectives of this grant proposal. Examination of gene expression patterns and characterization of possible down-stream genes are central to understanding the ets gene functions. In accordance with this understanding, two areas of experimental needs have been identified to be essential and can be best satisfied by core facilities. These are (A) DNA sequence analysis and (B) in situ detection of gene expression patterns. The objective of this core are: 1) To provide consolidated resource for DNA sequence analysis. 2) To provide standardized methodology and shared facilities for examining gene expression patterns.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA078582-04
Application #
6478161
Study Section
Project Start
2001-08-01
Project End
2002-07-31
Budget Start
Budget End
Support Year
4
Fiscal Year
2001
Total Cost
$76,744
Indirect Cost

  Institution

Name
Medical University of South Carolina
Department
Type
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425

  Publications

Scheiber, Melissa N; Watson, Patricia M; Rumboldt, Tihana et al. (2014) FLI1 expression is correlated with breast cancer cellular growth, migration, and invasion and altered gene expression. Neoplasia 16:801-13
Kornblau, Steven M; Qiu, Yi Hua; Zhang, Nianxiang et al. (2011) Abnormal expression of FLI1 protein is an adverse prognostic factor in acute myeloid leukemia. Blood 118:5604-12
Turner, David P; Findlay, Victoria J; Moussa, Omar et al. (2011) Mechanisms and functional consequences of PDEF protein expression loss during prostate cancer progression. Prostate 71:1723-35
Markiewicz, Margaret; Nakerakanti, Sashidhar S; Kapanadze, Bagrat et al. (2011) Connective tissue growth factor (CTGF/CCN2) mediates angiogenic effect of S1P in human dermal microvascular endothelial cells. Microcirculation 18:1-11
Findlay, Victoria J; Turner, David P; Yordy, John S et al. (2011) Prostate-Derived ETS Factor Regulates Epithelial-to-Mesenchymal Transition through Both SLUG-Dependent and Independent Mechanisms. Genes Cancer 2:120-9
Duchi, Serena; Fagnocchi, Luca; Cavaliere, Valeria et al. (2010) Drosophila VHL tumor-suppressor gene regulates epithelial morphogenesis by promoting microtubule and aPKC stability. Development 137:1493-503
Asano, Yoshihide; Stawski, Lukasz; Hant, Faye et al. (2010) Endothelial Fli1 deficiency impairs vascular homeostasis: a role in scleroderma vasculopathy. Am J Pathol 176:1983-98
Moussa, Omar; LaRue, Amanda C; Abangan Jr, Romeo S et al. (2010) Thrombocytopenia in mice lacking the carboxy-terminal regulatory domain of the Ets transcription factor Fli1. Mol Cell Biol 30:5194-206
Elkareh, Jihad; Periyasamy, Sankaridrug M; Shidyak, Amjad et al. (2009) Marinobufagenin induces increases in procollagen expression in a process involving protein kinase C and Fli-1: implications for uremic cardiomyopathy. Am J Physiol Renal Physiol 296:F1219-26
Shirai, Keisuke; Sera, Yasuhiko; Bulkeley, William et al. (2009) Hematopoietic stem cell origin of human fibroblasts: cell culture studies of female recipients of gender-mismatched stem cell transplantation and patients with chronic myelogenous leukemia. Exp Hematol 37:1464-71

Showing the most recent 10 out of 84 publications