The Microscopy Core will support each of the 4 projects with state-of-the-art electron microscopy, confocal microscopy and fluorescence microscopy equipment, services, and support. These will include: 1. Electron Microscopy and High Resolution Immunolocalization of Proteins. The Core will perform electron microscopic sample preparation and imaging as a service to the projects. Conventional embedded section microscopy will be available, and we are one of the few centers capable of resinless section EM. We have a stereo imaging capability for both techniques. Facilities for digitization of micrographs and for digital image processing will be provided. Training will be available for investigators wanting to do their own sample preparation and imaging. 2. Localization of Proteins and Nucleic Acids by Confocal Microscopy. The Microscopy Core will provide a dedicated Leica TCS SP1 microscope and will train users in its use. Several Leica SP2 confocals are available on a shared basis. Confocal microscopy as a service will be available to occasional users. Image processing software and training in its use will be provided. 3. Live Cell Imaging. The Core will assist users with the imaging of fluorescent proteins in living cells by both fluorescence and confocal microscopy. This will include time lapse studies, FRAP to measure mobility and binding, and FRET to detect protein-protein interactions. 4. Localization of Proteins and Nucleic Acids by Computer Deconvolution Microscopy. Each project will have access to the Digital Imaging Center. This center performs deconvolution of microscopy image stacks and provides sophisticated digital image processing and analysis. 5. Microscopy Consultation. The Core will provide assistance with experimental design, microscopy protocols and instruments, analysis of data, and the preparation of micrographs and figures for publication and presentation.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
5P01CA082834-10
Application #
8017466
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2010-02-01
Budget End
2011-01-31
Support Year
10
Fiscal Year
2010
Total Cost
$141,723
Indirect Cost
Name
University of Massachusetts Medical School Worcester
Department
Type
DUNS #
603847393
City
Worcester
State
MA
Country
United States
Zip Code
01655
Araya, H├ęctor F; Sepulveda, Hugo; Lizama, Carlos O et al. (2018) Expression of the ectodomain-releasing protease ADAM17 is directly regulated by the osteosarcoma and bone-related transcription factor RUNX2. J Cell Biochem 119:8204-8219
Carver, Gary E; Locknar, Sarah A; Weaver, Donald L et al. (2018) Real-time detection of breast cancer at the cellular level. J Cell Physiol :
Tracy, Kirsten M; Tye, Coralee E; Ghule, Prachi N et al. (2018) Mitotically-Associated lncRNA (MANCR) Affects Genomic Stability and Cell Division in Aggressive Breast Cancer. Mol Cancer Res 16:587-598
Zaidi, Sayyed K; Fritz, Andrew J; Tracy, Kirsten M et al. (2018) Nuclear organization mediates cancer-compromised genetic and epigenetic control. Adv Biol Regul 69:1-10
Hong, Deli; Fritz, Andrew J; Finstad, Kristiaan H et al. (2018) Suppression of Breast Cancer Stem Cells and Tumor Growth by the RUNX1 Transcription Factor. Mol Cancer Res 16:1952-1964
Zaidi, Sayyed K; Nickerson, Jeffrey A; Imbalzano, Anthony N et al. (2018) Mitotic Gene Bookmarking: An Epigenetic Program to Maintain Normal and Cancer Phenotypes. Mol Cancer Res 16:1617-1624
Hong, Deli; Fritz, Andrew J; Zaidi, Sayyed K et al. (2018) Epithelial-to-mesenchymal transition and cancer stem cells contribute to breast cancer heterogeneity. J Cell Physiol 233:9136-9144
Farina, Nicholas H; Zingiryan, Areg; Vrolijk, Michael A et al. (2018) Nanoparticle-based targeted cancer strategies for non-invasive prostate cancer intervention. J Cell Physiol 233:6408-6417
Tracy, Kirsten M; Tye, Coralee E; Page, Natalie A et al. (2018) Selective expression of long non-coding RNAs in a breast cancer cell progression model. J Cell Physiol 233:1291-1299
Hong, Deli; Fritz, Andrew J; Gordon, Jonathan A et al. (2018) RUNX1-dependent mechanisms in biological control and dysregulation in cancer. J Cell Physiol :

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