The goal of the RNA Sequencing and Data Analysis Core is to provide a centralized service for RNA sequencing and bioinformatics needs of the program. The benefits of this core include resources and expertise to perform RNA sequencing experiments, access to the most up-to-date equipment, kits and concepts, and cutting edge methods and tools for sequencing and integrative omics analyses. This team is particularly skilled in small RNA and mRNA (long) RNA sequencing and analyses, with emphasis on integration of these data with other -omics datasets. Many of the services provided by the Core have been developed and optimized at Vanderbilt University Medical Center (VUMC) by members of the leadership team, including cutting-edge protocols and analysis pipelines for small RNA sequencing of extracellular RNAs. The Core personnel have complementary expertise in biotechnology, bioinformatics, and biostatistics. The Core will 1) perform high throughput small RNA and total RNA sequencing, as well as other sequencing experiments; 2) provide multi-omics data analysis and interpretation; 3) develop novel integrative methods to discover key regulators in extracellular RNA biogenesis and function in colorectal cancer.

Public Health Relevance

The RNA Sequencing and Data Analysis Core will provide expertise and support for all projects in sequencing experiments, multi-omics (eg RNA-Seq and proteomics) data analysis, interpretation, data sharing and integration. We will perform small RNA and total RNA sequencing, provide state-of-the-art multi-level omics data, and develop novel integrative omics methods to provide a comprehensive understanding of exRNA biogenesis and function in colorectal cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Program Projects (P01)
Project #
1P01CA229123-01A1
Application #
9854708
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2020-01-01
Budget End
2020-12-31
Support Year
1
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
965717143
City
Nashville
State
TN
Country
United States
Zip Code
37203