Abstract

The GERO Cell Immortalization and Transfection Facility, or CIT, will be supervised by Dr. MacDougall as project leader. The Service Core is designed to serve the 4 scientific projects of the Research Core, and each project will require tooth specific and stably transfected cell lines. The Service Core will produce immortalized cell lines by gene transfer to several dental cell types in monolayer culture, through use of retroviral oncogene constructs. Stable cell lines will be isolated and gene expression profile and nature of extracellular matrix will be analyzed. These cell lines will serve as a resource for in vitro promoter studies in the Research Core projects. The Service Core will also perform stable cell transfections with various promoter-reporter constructs provided by the individual projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Program Projects (P01)
Project #
1P01DE013221-01A1
Application #
6203750
Study Section
Special Emphasis Panel (ZDE1)
Project Start
2000-04-01
Project End
2005-03-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2000
Total Cost
Indirect Cost

  Institution

Name
University of Texas Health Science Center San Antonio
Department
Type
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229

  Publications

Chen, S; Gluhak-Heinrich, J; Wang, Y H et al. (2009) Runx2, osx, and dspp in tooth development. J Dent Res 88:904-9
Hu, Yuanyuan; Papagerakis, Petros; Ye, Ling et al. (2008) Distal cis-regulatory elements are required for tissue-specific expression of enamelin (Enam). Eur J Oral Sci 116:113-23
Chen, Shuo; Chen, Lei; Jahangiri, Allen et al. (2008) Expression and processing of small integrin-binding ligand N-linked glycoproteins in mouse odontoblastic cells. Arch Oral Biol 53:879-89
Wittrant, Y; Bhandari, B Sriniketan; Abboud, H et al. (2008) PDGF up-regulates CSF-1 gene transcription in ameloblast-like cells. J Dent Res 87:33-8
Werner, S Abboud; Gluhak-Heinrich, J; Woodruff, K et al. (2007) Targeted expression of csCSF-1 in op/op mice ameliorates tooth defects. Arch Oral Biol 52:432-43
Paine, Michael L; Luo, Wen; Wang, Hong-Jun et al. (2005) Dentin sialoprotein and dentin phosphoprotein overexpression during amelogenesis. J Biol Chem 280:31991-8
Ye, Ling; MacDougall, Mary; Zhang, Shubin et al. (2004) Deletion of dentin matrix protein-1 leads to a partial failure of maturation of predentin into dentin, hypomineralization, and expanded cavities of pulp and root canal during postnatal tooth development. J Biol Chem 279:19141-8
Zhang, J H; Wang, J; Tang, J et al. (2004) Bone sialoprotein promotes bone metastasis of a non-bone-seeking clone of human breast cancer cells. Anticancer Res 24:1361-8
Tu, Qisheng; Yamauchi, Masato; Pageau, Steven C et al. (2004) Autoregulation of bone sialoprotein gene in pre-osteoblastic and non-osteoblastic cells. Biochem Biophys Res Commun 316:461-7
Zhang, Jian-Hua; Tang, Jean; Wang, Jie et al. (2003) Over-expression of bone sialoprotein enhances bone metastasis of human breast cancer cells in a mouse model. Int J Oncol 23:1043-8

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