Core A measures serum, serum ultrafiltrate, plasma and urine stone risk chemistries and calculates urine supersaturations for patients studied in Projects 1 and 2. These measurements are used for fulfillment of aims within all four central program project objectives. Upper limit of metastability (ULM) for calcium oxalate and calcium phosphate in urine will be measured for Project 1. Core A will also measure plasma renin for Project 1. Blood and urine samples are processed and shipped for assay of lithium done in Core B, measurement of inflammatory markers in Core D and measurement of intact parathyroid hormone (PTH), aldosterone, 25(OH)- vitamin D and 1,25-dihydroxyvitamin D (performed by LabCorp). Urine exosomes will be prepared for molecular expression studies of renal ion transporter proteins. Aliquots of all blood and urine specimens are stored at -80C for future use. All laboratory data from Core A for all projects are consolidated into a single database with daily backup for security and ease of analysis. Ultimately, data from the other projects and cores (e.g. histology, biopsy, stone analysis etc.) will be consolidated into our database so that it can be analyzed along with serum and urine data.
Core A performs blood and urine assays and renal ion transporter studies to characterize the abnormalities of renal calcium reabsorption that lead to renal papillary calcifications and calcium stone formation. Data from Core A will help in establishing links between metabolic abnormalities (Project 1), histopathology (Project 3), and renal papillary disease phenotype (Project 2), as well as understanding mechanisms of action of therapies commonly used to treat stone disease.
| Williams Jr, James C; Borofsky, Michael S; Bledsoe, Sharon B et al. (2018) Papillary Ductal Plugging is a Mechanism for Early Stone Retention in Brushite Stone Disease. J Urol 199:186-192 |
| Worcester, Elaine M; Bergsland, Kristin J; Gillen, Daniel L et al. (2018) Mechanism for higher urine pH in normal women compared with men. Am J Physiol Renal Physiol 314:F623-F629 |
| Bergsland, Kristin J; Coe, Fredric L; Parks, Joan H et al. (2018) Evidence for a role of PDZ domain-containing proteins to mediate hypophosphatemia in calcium stone formers. Nephrol Dial Transplant 33:759-770 |
| Kleinguetl, Colin; Williams Jr, James C; Ibrahim, Samar A et al. (2017) Calcium Tartrate Tetrahydrate, Case Report of a Novel Human Kidney Stone. J Endourol Case Rep 3:192-195 |
| Mulay, Shrikant R; Eberhard, Jonathan N; Desai, Jyaysi et al. (2017) Hyperoxaluria Requires TNF Receptors to Initiate Crystal Adhesion and Kidney Stone Disease. J Am Soc Nephrol 28:761-768 |
| Winfree, Seth; Khan, Shehnaz; Micanovic, Radmila et al. (2017) Quantitative Three-Dimensional Tissue Cytometry to Study Kidney Tissue and Resident Immune Cells. J Am Soc Nephrol 28:2108-2118 |
| Borofsky, Michael S; Dauw, Casey A; York, Nadya et al. (2017) Accuracy of daily fluid intake measurements using a ""smart"" water bottle. Urolithiasis : |
| Winfree, Seth; Ferkowicz, Michael J; Dagher, Pierre C et al. (2017) Large-scale 3-dimensional quantitative imaging of tissues: state-of-the-art and translational implications. Transl Res 189:1-12 |
| Cohen, Andrew J; Borofsky, Michael S; Anderson, Blake B et al. (2017) Endoscopic Evidence That Randall's Plaque is Associated with Surface Erosion of the Renal Papilla. J Endourol 31:85-90 |
| Gilad, Ron; Williams Jr, James C; Usman, Kalba D et al. (2017) Interpreting the results of chemical stone analysis in the era of modern stone analysis techniques. J Nephrol 30:135-140 |
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