Core B will conduct all the morphological and pathology analyses for Projects 1, 2, and 3. Papillary scoring is an essential part of the PPG, and Core B will analyze all surgical recordings from Projects 1 and 2 to quantify plaque and plug areas on all papillae and to ensure that papillary scoring across the projects is consistent. Human tissues will be collected by Project 2 from carefully categorized stone patients, and Core B will perform light and electron microscopy (LM and EM) using specialized stains on the biopsy material, and using micro- infrared spectroscopy (FT-IR) and Raman methods to identify mineral in the tissue. Core B will also prepare the frozen sections to be used by Project 3 for laser micro-dissection, along with immunostained sections to verify the biopsy content. All stones collected in Projects 1 and 2 will be analyzed by micro CT and conventional FT-IR to provide detailed information on stone type and percent mineral content. Core B will also perform atomic absorption spectrophotometric analysis of lithium to determine distal delivery of solutes in study patients (Project 1), and of calcium in tissue specimens (Project 3).
Our long-term goal is to discover the mechanisms underlying different forms of kidney stone disease, so that effective treatments can be developed for this common but pathologically diverse condition.
| Williams Jr, James C; Borofsky, Michael S; Bledsoe, Sharon B et al. (2018) Papillary Ductal Plugging is a Mechanism for Early Stone Retention in Brushite Stone Disease. J Urol 199:186-192 |
| Worcester, Elaine M; Bergsland, Kristin J; Gillen, Daniel L et al. (2018) Mechanism for higher urine pH in normal women compared with men. Am J Physiol Renal Physiol 314:F623-F629 |
| Bergsland, Kristin J; Coe, Fredric L; Parks, Joan H et al. (2018) Evidence for a role of PDZ domain-containing proteins to mediate hypophosphatemia in calcium stone formers. Nephrol Dial Transplant 33:759-770 |
| Kleinguetl, Colin; Williams Jr, James C; Ibrahim, Samar A et al. (2017) Calcium Tartrate Tetrahydrate, Case Report of a Novel Human Kidney Stone. J Endourol Case Rep 3:192-195 |
| Mulay, Shrikant R; Eberhard, Jonathan N; Desai, Jyaysi et al. (2017) Hyperoxaluria Requires TNF Receptors to Initiate Crystal Adhesion and Kidney Stone Disease. J Am Soc Nephrol 28:761-768 |
| Winfree, Seth; Khan, Shehnaz; Micanovic, Radmila et al. (2017) Quantitative Three-Dimensional Tissue Cytometry to Study Kidney Tissue and Resident Immune Cells. J Am Soc Nephrol 28:2108-2118 |
| Borofsky, Michael S; Dauw, Casey A; York, Nadya et al. (2017) Accuracy of daily fluid intake measurements using a ""smart"" water bottle. Urolithiasis : |
| Winfree, Seth; Ferkowicz, Michael J; Dagher, Pierre C et al. (2017) Large-scale 3-dimensional quantitative imaging of tissues: state-of-the-art and translational implications. Transl Res 189:1-12 |
| Cohen, Andrew J; Borofsky, Michael S; Anderson, Blake B et al. (2017) Endoscopic Evidence That Randall's Plaque is Associated with Surface Erosion of the Renal Papilla. J Endourol 31:85-90 |
| Gilad, Ron; Williams Jr, James C; Usman, Kalba D et al. (2017) Interpreting the results of chemical stone analysis in the era of modern stone analysis techniques. J Nephrol 30:135-140 |
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