Abstract

The overall objective of the Mouse Core is to provide for the maintenance of the genotoxicant reporter mouse colonies that will be used by the Projects for their experiments. The Mouse Core will maintain the mouse colonies that will be used by the projects for their experiments. The Mouse Core will maintain the mouse colonies, carry out breeding programs for the production of experimental animals in coordination with each project leader, obtain samples for genotyping during the first week after birth, and cull the unwanted animals before weaning in order to minimize the size and cost of maintaining each line. The Mouse Core will closely monitor the health of the animals and look for the presence of abnormal phenotypes such as the presence of tumors. Extensive recordkeeping will be kept on spread sheets for each line sot that pedigree can be established when indicated. Periodic status reports will be provided to Project leaders. Routine and simple experimental procedures that can be done in the procedural room adjacent to the animal room will be performed by Mouse Core personnel. Routine necropsy and preservation of tissue will be performed by Mouse Core personnel in coordination with Project leaders and other Cores.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program Projects (P01)
Project #
2P01ES005652-07
Application #
6239579
Study Section
Project Start
1997-09-01
Project End
1998-08-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
7
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Cincinnati
Department
Type
DUNS #
City
Cincinnati
State
OH
Country
United States
Zip Code
45221

  Publications

Barrera-Oro, Julio; Liu, Tzu-Yang; Gorden, Erin et al. (2008) Role of the mismatch repair gene, Msh6, in suppressing genome instability and radiation-induced mutations. Mutat Res 642:74-9
Hersh, Megan N; Stambrook, Peter J; Stringer, James R (2002) Visualization of mosaicism in tissues of normal and mismatch-repair-deficient mice carrying a microsatellite-containing transgene. Mutat Res 505:51-62
Shao, Changshun; Yin, Moying; Deng, Li et al. (2002) Loss of heterozygosity and point mutation at Aprt locus in T cells and fibroblasts of Pms2-/- mice. Oncogene 21:2840-5
Cervantes, Rachel B; Stringer, James R; Shao, Changshun et al. (2002) Embryonic stem cells and somatic cells differ in mutation frequency and type. Proc Natl Acad Sci U S A 99:3586-90
Mitchell, K R; Warshawsky, D (2001) Comparison of Ha-ras mutational spectra of N-methyldibenzo[c,g]carbazole and 7H-dibenzo[c,g]carbazole-induced mouse skin tumors. Mol Carcinog 32:55-60
Xue, W; Schneider, J; Mitchell, K et al. (2001) trans-3,4-dihydroxy-anti-1,2-epoxy-1,2,3,4-tetrahydrodi- benz[a,j]acridine involvement in dibenz[a,j]acridine DNA adduct formation in mouse skin consistent with Ha-ras mutation patterns in tumors. Chem Res Toxicol 14:871-8
Shao, C; Stambrook, P J; Tischfield, J A (2001) Mitotic recombination is suppressed by chromosomal divergence in hybrids of distantly related mouse strains. Nat Genet 28:169-72
Nikiforova, M N; Stringer, J R; Blough, R et al. (2000) Proximity of chromosomal loci that participate in radiation-induced rearrangements in human cells. Science 290:138-41
Conn, C W; Hennigan, R F; Dai, W et al. (2000) Incomplete cytokinesis and induction of apoptosis by overexpression of the mammalian polo-like kinase, Plk3. Cancer Res 60:6826-31
Rose, J A; Yates, P A; Simpson, J et al. (2000) Biallelic methylation and silencing of mouse Aprt in normal kidney cells. Cancer Res 60:3404-8

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