Abstract

cells. We will perform microarray, epigenotyping, and bioinformatic analysis of these clones, fully reprogrammed """"""""human ES cell""""""""-like cells, wild type human ES cells, and mesenchymal and myeloid cells to offer insights into reprogramming to a pluripotent state and into cell fate changes to other lineages. This project will result in a significant improvement in our understanding of how the developmental status of differentiated cells can be altered. Reprogramming the genome from a somatic cell to a pluripotent state would be useful to generate patient-specific ES cells, thus overcoming the problem of tissue rejection resulting from genetic mismatches between donor and recipient. Perhaps more importantly, understanding how to reprogram differentiated cells to entirely new fates may ultimately lead to novel regenerative therapies that act on patients'endogenous cells in the absence of cell transplantation. This project represents an important early step in that direction.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
7P01GM081629-04
Application #
8338839
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2011-08-01
Budget End
2012-07-31
Support Year
4
Fiscal Year
2011
Total Cost
$410,814
Indirect Cost

  Institution

Name
Morgridge Institute for Research, Inc.
Department
Type
DUNS #
012420082
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Rytz, Thérèse C; Miller, Marcus J; McLoughlin, Fionn et al. (2018) SUMOylome Profiling Reveals a Diverse Array of Nuclear Targets Modified by the SUMO Ligase SIZ1 during Heat Stress. Plant Cell 30:1077-1099
Kumar, Akhilesh; D'Souza, Saritha Sandra; Moskvin, Oleg V et al. (2017) Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts. Cell Rep 19:1902-1916
Aguilar-Hernández, Victor; Kim, Do-Young; Stankey, Robert J et al. (2017) Mass Spectrometric Analyses Reveal a Central Role for Ubiquitylation in Remodeling the Arabidopsis Proteome during Photomorphogenesis. Mol Plant 10:846-865
Suknuntha, Kran; Ishii, Yuki; Tao, Lihong et al. (2015) Discovery of survival factor for primitive chronic myeloid leukemia cells using induced pluripotent stem cells. Stem Cell Res 15:678-693
Shortreed, Michael R; Wenger, Craig D; Frey, Brian L et al. (2015) Global Identification of Protein Post-translational Modifications in a Single-Pass Database Search. J Proteome Res 14:4714-20
Sheynkman, Gloria M; Shortreed, Michael R; Frey, Brian L et al. (2014) Large-scale mass spectrometric detection of variant peptides resulting from nonsynonymous nucleotide differences. J Proteome Res 13:228-40
Brumbaugh, Justin; Russell, Jason D; Yu, Pengzhi et al. (2014) NANOG is multiply phosphorylated and directly modified by ERK2 and CDK1 in vitro. Stem Cell Reports 2:18-25
Bailey, Derek J; McDevitt, Molly T; Westphall, Michael S et al. (2014) Intelligent data acquisition blends targeted and discovery methods. J Proteome Res 13:2152-61
Ladror, Daniel T; Frey, Brian L; Scalf, Mark et al. (2014) Methylation of yeast ribosomal protein S2 is elevated during stationary phase growth conditions. Biochem Biophys Res Commun 445:535-41
Sheynkman, Gloria M; Johnson, James E; Jagtap, Pratik D et al. (2014) Using Galaxy-P to leverage RNA-Seq for the discovery of novel protein variations. BMC Genomics 15:703

Showing the most recent 10 out of 47 publications