Abstract

The goal of this core facility is to provide the technical support for the performance of the variety of RIAs required by the individual projects of the Program. The rationale for establishing this core is multifold. First, we will centralize most of the RIAs in one laboratory. Second, we will not duplicate supplies and efforts. Third, the data generated by this core facility will foster scientific exchange between individual laboratories. Lastly, the core director has over fifteen years of experience in developing and using RIAs specific for POMC-derive peptides, as well as many other peptide hormones. Virtually all of the studies within measure the POMC-derived peptides, ACTH, beta-endorphin, the alpha and beta-melanocyte stimulating hormones (CLIP) corticotropin- like-intermediate-lobe-peptide, as well as assist Project VI assaying CRH, SST, ANP, AVP and oxytocin.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD030236-03
Application #
5212843
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

New, M I; Nimkarn, S; Brandon, D D et al. (2001) Resistance to multiple steroids in two sisters. J Steroid Biochem Mol Biol 76:161-6
Slugg, R M; Hayward, M D; Ronnekleiv, O K et al. (2000) Effect of the mu-opioid agonist DAMGO on medial basal hypothalamic neurons in beta-endorphin knockout mice. Neuroendocrinology 72:208-17
Samuels, M H; Brandon, D D; Isabelle, L M et al. (2000) Cortisol production rates in subjects with suspected Cushing's syndrome: assessment by stable isotope dilution methodology and comparison to other diagnostic methods. J Clin Endocrinol Metab 85:22-8
Coste, S C; Kesterson, R A; Heldwein, K A et al. (2000) Abnormal adaptations to stress and impaired cardiovascular function in mice lacking corticotropin-releasing hormone receptor-2. Nat Genet 24:403-9
Grahame, N J; Mosemiller, A K; Low, M J et al. (2000) Naltrexone and alcohol drinking in mice lacking beta-endorphin by site-directed mutagenesis. Pharmacol Biochem Behav 67:759-66
Grisel, J E; Mogil, J S; Grahame, N J et al. (1999) Ethanol oral self-administration is increased in mutant mice with decreased beta-endorphin expression. Brain Res 835:62-7
Brandon, D D; Isabelle, L M; Samuels, M H et al. (1999) Cortisol production rate measurement by stable isotope dilution using gas chromatography-negative ion chemical ionization mass spectrometry. Steroids 64:372-8
New, M I; Nimkarn, S; Brandon, D D et al. (1999) Resistance to several steroids in two sisters. J Clin Endocrinol Metab 84:4454-64
Grahame, N J; Low, M J; Cunningham, C L (1998) Intravenous self-administration of ethanol in beta-endorphin-deficient mice. Alcohol Clin Exp Res 22:1093-8
Abrami, L; Fivaz, M; Decroly, E et al. (1998) The pore-forming toxin proaerolysin is activated by furin. J Biol Chem 273:32656-61

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