The program project """""""" Genital Tract in HIV pathogenesis"""""""" will investigate immunologic, and host factors in the female genital microenvironment that influence HIV infectivity and susceptibility. This program project integrates three research projects and three Core facilities. Subproject 0001, """"""""Bacterial Vaginosis ( BV) and HIV in the Female Genital Tract"""""""" proposes that BV in the genital tract increases HIV susceptibility and will assess the effects or organisms associated with BV on HIV expression. Subproject 0002, """"""""Female Genital Tract Immunity and HIV vaccine responses"""""""" focuses on HIV specific vaccination, and the role of pre-existing genital tract HIV immunity in the response to immunization. Subproject 0002, """"""""HIV-1 Susceptibility Factors in Cervical Secretions"""""""" will focus on the microbiology/immunology of the cervical microenvironment and how secreted and microbial factors inhibit or enhance susceptibility to HIV infection in the female genital tract. The projects will utilize the well established HIV cohorts (REACH, WIHS, WITS) spanning ages from adolescence to adulthood and the spectrum of HIV infection from asymptomatic to AIDS as well as two nigh risk seronegative cohorts. The research projects are supported by three Cores: Statistics and Data Management Core coordinating research, scientific meetings and data management support; the Clinical Core managing subject recruitment, existing cohort utilization and clinical data collection; and the Laboratory Core establishing a specimen repository and providing virologic and immunologic assays. By characterizing factors affecting HIV infectivity and susceptibility to infection, the studies will increase our understanding of sexual and perinatal HIV transmission and contribute to future therapy and vaccine strategies.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
1P01HD040539-01
Application #
6331673
Study Section
Special Emphasis Panel (ZHD1-DSR-R (06))
Program Officer
Reichelderfer, Patricia
Project Start
2001-04-23
Project End
2006-03-31
Budget Start
2001-04-23
Budget End
2002-03-31
Support Year
1
Fiscal Year
2001
Total Cost
$1,065,108
Indirect Cost
Name
Rush University Medical Center
Department
Type
DUNS #
City
Chicago
State
IL
Country
United States
Zip Code
60612
Spear, Gregory T; Kendrick, Sabrina R; Chen, Hua Y et al. (2011) Multiplex immunoassay of lower genital tract mucosal fluid from women attending an urban STD clinic shows broadly increased IL1ß and lactoferrin. PLoS One 6:e19560
Cook, Judith A (2011) Associations between use of crack cocaine and HIV-1 disease progression: research findings and implications for mother-to-infant transmission. Life Sci 88:931-9
St John, Elizabeth P; Zariffard, M Reza; Martinson, Jeffrey A et al. (2009) Effect of mucosal fluid from women with bacterial vaginosis on HIV trans-infection mediated by dendritic cells. Virology 385:22-7
Anzinger, Joshua J; Olinger, Gene G; Spear, Gregory T (2008) Donor variability in HIV binding to peripheral blood mononuclear cells. Virol J 5:95
Spear, Gregory T; Sikaroodi, Masoumeh; Zariffard, M Reza et al. (2008) Comparison of the diversity of the vaginal microbiota in HIV-infected and HIV-uninfected women with or without bacterial vaginosis. J Infect Dis 198:1131-40
Spear, Gregory T; Zariffard, M Reza; Cohen, Mardge H et al. (2008) Vaginal IL-8 levels are positively associated with Candida albicans and inversely with lactobacilli in HIV-infected women. J Reprod Immunol 78:76-9
Mares, Debra; Simoes, Jose A; Novak, Richard M et al. (2008) TLR2-mediated cell stimulation in bacterial vaginosis. J Reprod Immunol 77:91-9
St John, Elizabeth; Mares, Debra; Spear, Gregory T (2007) Bacterial vaginosis and host immunity. Curr HIV/AIDS Rep 4:22-8
Novak, Richard M; Donoval, Betty A; Graham, Parrie J et al. (2007) Cervicovaginal levels of lactoferrin, secretory leukocyte protease inhibitor, and RANTES and the effects of coexisting vaginoses in human immunodeficiency virus (HIV)-seronegative women with a high risk of heterosexual acquisition of HIV infection. Clin Vaccine Immunol 14:1102-7
St John, Elizabeth P; Martinson, Jeff; Simoes, Jose A et al. (2007) Dendritic cell activation and maturation induced by mucosal fluid from women with bacterial vaginosis. Clin Immunol 125:95-102

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