Abstract

Core III produces and maintains cell culture and disaggregated single cell preparations for the Projects of this Program. The Core also treats preparations as requested by the individual Projects to serve the needs of those Projects (e.g. sustained growth in peptides or catecholamines; adenovirus transfection with specific plasmids). As such, the Core serves a number of functions. First, it relieves the Projects of the cost and time of maintaining and operating individual cell disaggregation and culture facilities. All equipment, supplies and supporting staff are centralized both physically and administratively. Second, it provides the projects with a source of technical expertise when planning experimental designs around cell cultures and single cells. Third, the Core works with Projects to develop and characterize new cell preparations, as required by evolving experimental results.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL028958-18
Application #
6335053
Study Section
Project Start
2000-07-01
Project End
2001-06-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
18
Fiscal Year
2000
Total Cost
$294,128
Indirect Cost

  Institution

Name
Columbia University (N.Y.)
Department
Type
DUNS #
167204994
City
New York
State
NY
Country
United States
Zip Code
10032

  Publications

Almaça, Joana; Liang, Tao; Gaisano, Herbert Y et al. (2015) Spatial and temporal coordination of insulin granule exocytosis in intact human pancreatic islets. Diabetologia 58:2810-8
Nawathe, Pooja A; Kryukova, Yelena; Oren, Ronit V et al. (2013) An LQTS6 MiRP1 mutation suppresses pacemaker current and is associated with sinus bradycardia. J Cardiovasc Electrophysiol 24:1021-7
Kryukova, Yelena N; Protas, Lev; Robinson, Richard B (2012) Ca2+-activated adenylyl cyclase 1 introduces Ca2+-dependence to beta-adrenergic stimulation of HCN2 current. J Mol Cell Cardiol 52:1233-9
Yan, Qinghong; Masson, Rajeev; Ren, Yi et al. (2012) Evolution of CpG island promoter function underlies changes in KChIP2 potassium channel subunit gene expression in mammalian heart. Proc Natl Acad Sci U S A 109:1601-6
Guo, Jianfen; Gertsberg, Zoya; Ozgen, Nazira et al. (2011) Protein kinase D isoforms are activated in an agonist-specific manner in cardiomyocytes. J Biol Chem 286:6500-9
Zhang, Hao; Lau, David H; Shlapakova, Iryna N et al. (2011) Implantation of sinoatrial node cells into canine right ventricle: biological pacing appears limited by the substrate. Cell Transplant 20:1907-14
Rosati, Barbara; Yan, Qinghong; Lee, Mi Sun et al. (2011) Robust L-type calcium current expression following heterozygous knockout of the Cav1.2 gene in adult mouse heart. J Physiol 589:3275-88
Kanaporis, G; Brink, P R; Valiunas, V (2011) Gap junction permeability: selectivity for anionic and cationic probes. Am J Physiol Cell Physiol 300:C600-9
Potapova, Irina A; Cohen, Ira S; Doronin, Sergey V (2010) Von willebrand factor increases endothelial cell adhesiveness for human mesenchymal stem cells by activating p38 mitogen-activated protein kinase. Stem Cell Res Ther 1:35
Wang, Wei; Gao, Junyuan; Entcheva, Emilia et al. (2010) A transmural gradient in the cardiac Na/K pump generates a transmural gradient in Na/Ca exchange. J Membr Biol 233:51-62

Showing the most recent 10 out of 303 publications