We wish to provide a central electron microscopy laboratory for the use of members of the program project. We propose to continue using the existing EM lab in the Department of Molecular Genetics and Cell Biology as the core lab for this purpose. As in the past the Core Lab will be a central facility providing training and materials for members of the Program Project. We have operated the lab effectively as a shared instrumentation facility and extending access to members of the Program Project has been a natural outgrowth of this philosophy. The laboratory currently contain a Philips EM 300 in excellent condition and is in the process of acquiring a Philips CM10 electron microscope, including a low dose kit and a Gatan cryospecimen holder for the Philips CM10. In addition, the laboratory contains other equipment normally used in an electron microscopy laboratory (described in the facilities available statement). The day-to-day operation of the lab is under the direction of our very competent departmental electron microscope technologist, Mr. Gerald Grofman. The overall administration of the lab will continue to be the responsibility of Dr. Robert Josephs. We have begun a program to use cryoelectron microscopy in the Core Lab. Appropriate computer image processing procedures have been implemented and a device for rapid freezing of specimens has been fabricated. These will be used in conjunction with the research proposed in Project I, II, and VII.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL030121-10
Application #
3780326
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of Chicago
Department
Type
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637
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