The function of the BIOCHEMISTRY CORE is to provide instrumentation, facilities and technical expertise for performing routine, state-of-the-art assays of the many lipid species that are of interest to each of the program projects. The success of all three individual projects is dependent on the ready availability of high quality analytical assays, using standardized protocols, for the quantitative determination of the individual lipid species that are central to understanding the biochemical and biological processes under investigation. Performance of these biochemical assays in a centralized facility, rather than in each individual laboratory, is an efficient and economical means for maintaining and using state-of-the-art expertise and instruments for lipid analysis. As necessary to address the specific aims of the individual projects, an additional function of the Core is to develop and implement new analytical methods, generally employing mass spectrometric detection, to measure lipid species that are either novel or difficult to assay by existing approaches. The Core, having recently added a workstation and software for more efficient data processing and multivariate statistical analysis, also is a resource to assist the individual projects with sophisticated processing, evaluation and interpretation of complex data.
| Zemski Berry, Karin A; Murphy, Robert C; Kosmider, Beata et al. (2017) Lipidomic characterization and localization of phospholipids in the human lung. J Lipid Res 58:926-933 |
| Mould, Kara J; Barthel, Lea; Mohning, Michael P et al. (2017) Cell Origin Dictates Programming of Resident versus Recruited Macrophages during Acute Lung Injury. Am J Respir Cell Mol Biol 57:294-306 |
| Gibbings, Sophie L; Thomas, Stacey M; Atif, Shaikh M et al. (2017) Three Unique Interstitial Macrophages in the Murine Lung at Steady State. Am J Respir Cell Mol Biol 57:66-76 |
| Frasch, S Courtney; McNamee, Eóin N; Kominsky, Douglas et al. (2016) G2A Signaling Dampens Colitic Inflammation via Production of IFN-?. J Immunol 197:1425-34 |
| Janssen, William J; Bratton, Donna L; Jakubzick, Claudia V et al. (2016) Myeloid Cell Turnover and Clearance. Microbiol Spectr 4: |
| Yun, Bogeon; Lee, HeeJung; Jayaraja, Sabarirajan et al. (2016) Prostaglandins from Cytosolic Phospholipase A2?/Cyclooxygenase-1 Pathway and Mitogen-activated Protein Kinases Regulate Gene Expression in Candida albicans-infected Macrophages. J Biol Chem 291:7070-86 |
| Desch, A Nicole; Gibbings, Sophie L; Goyal, Rajni et al. (2016) Flow Cytometric Analysis of Mononuclear Phagocytes in Nondiseased Human Lung and Lung-Draining Lymph Nodes. Am J Respir Crit Care Med 193:614-26 |
| Jayaraja, Sabarirajan; Dakhama, Azzeddine; Yun, Bogeon et al. (2016) Cytosolic phospholipase A2 contributes to innate immune defense against Candida albicans lung infection. BMC Immunol 17:27 |
| Kandasamy, Pitchaimani; Numata, Mari; Berry, Karin Zemski et al. (2016) Structural analogs of pulmonary surfactant phosphatidylglycerol inhibit toll-like receptor 2 and 4 signaling. J Lipid Res 57:993-1005 |
| Zemski Berry, Karin A; Murphy, Robert C (2016) Phospholipid Ozonation Products Activate the 5-Lipoxygenase Pathway in Macrophages. Chem Res Toxicol 29:1355-64 |
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