The objective of this Component is to provide extensive tissue culture support for storage of cell lines, for the large scale culture of individual lines required for protein purification or isolation of cell- specific protein factors, for immortalization of lymphocytes for human genetics studies, and to provide embryonic stem cells and feeder layer cultures for gene targeting studies. This Core will be expanded to support 20% of a senior cardiovascular researcher who will analyze heart function in our transgenic, knockout and gene replacement mice. This will make possible the detailed and quantitative analysis of physiological and pharmacological characteristics of knockout or gene replacement mice, including heart and vascular smooth muscle.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL041496-12
Application #
6202286
Study Section
Project Start
1999-12-01
Project End
2001-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
12
Fiscal Year
2000
Total Cost
$206,737
Indirect Cost
Name
University of Cincinnati
Department
Type
DUNS #
041064767
City
Cincinnati
State
OH
Country
United States
Zip Code
45221
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