The function of this core unit is to assist investigators of this PPG in the in vivo functional evaluation of human cells transduced by their vectors. This will be achieved by using transplantations of transduced human hemopoietic cells into a receptive xenogeneic host (NOD/SCID or NOD/SCID/beta2/mug-/-). The unit will generate and maintain sufficient numbers of NOD/SCID or NOD/SCID/beta2/mug-/- mice, to accommodate the needs of investigators of this PPG, and will be responsible for the mechanics of transplantation (i.e., irradiation, i.v., or intramarrow injection of donor cells). In addition, the unit will undertake the sampling and assist, at least in part, in the post-transplant evaluation required to assess the fate of the transplanted human cells.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL053750-12
Application #
7124277
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2005-09-01
Budget End
2006-08-31
Support Year
12
Fiscal Year
2005
Total Cost
$104,040
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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