Abstract

The function of the animal core is to provide services in many aspects of this program project, including Projects 1, 2, 4, and 5. First, it will supply the animals which produce fertilized ovum and foster mothers for transgenic experiments. It will also provide ES cells and blastocysts for the homologous recombination experiments. It will monitor the quality of the ES cells used and maintain the transgenic and homologous recombination mouse lines and breed the different lines to homozygosity in order to create a mouse model which carries human sickle hemoglobin as the predominant adult hemoglobin. It will maintain these animals and provide them to other investigators for testing of pharmacological agents.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL053762-05
Application #
6110436
Study Section
Project Start
1998-09-01
Project End
2000-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
5
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Mizunoe, Shota; Shuto, Tsuyoshi; Suzuki, Shingo et al. (2012) Synergism between interleukin (IL)-17 and Toll-like receptor 2 and 4 signals to induce IL-8 expression in cystic fibrosis airway epithelial cells. J Pharmacol Sci 118:512-520
Ye, Lin; Chang, Judy C; Lu, Ronghua et al. (2008) High oxygen environment during pregnancy rescues sickle cell anemia mice from prenatal death. Blood Cells Mol Dis 41:67-72
Han, Xiao-Dong; Lin, Chin; Chang, Judy et al. (2007) Fetal gene therapy of alpha-thalassemia in a mouse model. Proc Natl Acad Sci U S A 104:9007-11
Chang, Judy C; Ye, Lin; Kan, Yuet Wai (2006) Correction of the sickle cell mutation in embryonic stem cells. Proc Natl Acad Sci U S A 103:1036-40
Jimenez, D F; Lee, C I; O'Shea, C E et al. (2005) HIV-1-derived lentiviral vectors and fetal route of administration on transgene biodistribution and expression in rhesus monkeys. Gene Ther 12:821-30
Jimenez, Daniel F; Leapley, Alyssa C; Lee, Chang I et al. (2005) Fetal CD34+ cells in the maternal circulation and long-term microchimerism in rhesus monkeys (Macaca mulatta). Transplantation 79:142-6
Jimenez, Daniel F; Tarantal, Alice F (2003) Quantitative analysis of male fetal DNA in maternal serum of gravid rhesus monkeys (Macaca mulatta). Pediatr Res 53:18-23
Meiering, C D; Comstock, K E; Linial, M L (2000) Multiple integrations of human foamy virus in persistently infected human erythroleukemia cells. J Virol 74:1718-26
Lipshutz, G S; Sarkar, R; Flebbe-Rehwaldt, L et al. (1999) Short-term correction of factor VIII deficiency in a murine model of hemophilia A after delivery of adenovirus murine factor VIII in utero. Proc Natl Acad Sci U S A 96:13324-9
Lipshutz, G S; Flebbe-Rehwaldt, L; Gaensler, K M (1999) Adenovirus-mediated gene transfer to the peritoneum and hepatic parenchyma of fetal mice in utero. Surgery 126:171-7

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