The purpose of the Tissue Processing and Cell Culture Core (Core C) component of this PPG is to providecentralized expert handling of clinical samples obtained from human subjects by the Clinical Core B. Thetissue processing and cell culture activities of Core C will convert the clinical samples into materials (airwaycells, fluid, slides, etc.) required for the research proposed in all three Projects. This core will also utilizeexplanted lungs to culture and provide human primary smooth muscle and epithelial cells to the projects inthis PPG. There are many advantages to having these activities done by a single Core facility rather than ineach investigator's own laboratory. Foremost, the personnel staffing of the Core are highly experienced inperforming the required procedures in a meticulous and standardized manner.In addition Core C will be responsible for ensuring that samples are properly characterized, inventoried,stored, and that data is mantained in a data base with appropriate access to PPG investigators and othercores. In this capacity, the Core will allow for each clinical sample to be utilized to its full potential inmultiple Projects. This Core will interact closely with all Cores in this PPG (Core A, B and D) to maintainthe extensive database required to catalog and manage the processed clinical materials and generatecoherent links among experimental results obtained with these materials.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
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Heart, Lung, and Blood Initial Review Group (HLBP)
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Cleveland Clinic Lerner
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